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AB175647

FITC Anti-c-Fos antibody [H15-S]

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(2 Publications)

Rabbit Monoclonal c-Fos antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FOS.

View Alternative Names

G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

1 Images
Flow Cytometry - FITC Anti-c-Fos antibody [H15-S] (AB175647)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-c-Fos antibody [H15-S] (AB175647)

Flow cytometric analysis of permeabilized Human peripheral blood lymphocytes labeling c-Fos with ab175647 at 10μl/1 x 106 cells (blue) compared with an isotype control (black).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

H15-S

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human FOS. The exact immunogen used to generate this antibody is proprietary information.

P01100

Epitope

Antibody detects the linear epitope at the C-terminus of human c-FOS

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(in a 100 μl experimental sample).</p>" } } }
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Product details

The monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
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Properties and storage information

Form
Liquid
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Storage buffer
pH: 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.33% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
See full target information FOS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34939923

2021

External signals regulate continuous transcriptional states in hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eva M Fast,Audrey Sporrij,Margot Manning,Edroaldo Lummertz Rocha,Song Yang,Yi Zhou,Jimin Guo,Ninib Baryawno,Nikolaos Barkas,David Scadden,Fernando Camargo,Leonard I Zon

Cancers 13: PubMed34945008

2021

Deep Phenotypic Characterisation of CTCs by Combination of Microfluidic Isolation (IsoFlux) and Imaging Flow Cytometry (ImageStream).

Applications

Unspecified application

Species

Unspecified reactive species

Antonio J Ruiz-Rodríguez,Maria P Molina-Vallejo,Inés Aznar-Peralta,Cristina González Puga,Inés Cañas García,Encarna González,Jose A Lorente,M Jose Serrano,M Carmen Garrido-Navas
View all publications
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