AB4223

FITC Anti-C1q antibody

Name: FITC conjugated Anti-C1q antibody (ab4223) | Abcam
Brand: Abcam Limited
SKU: AB4223
Price: 585 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(15 Publications)

Anti-C1q antibody - FITC conjugated (ab4223) is a rabbit polyclonal antibody detecting C1q in Flow Cytometry, IHC-P. Suitable for Human.

- Trusted since 2003

View Alternative Names

Complement C1q subcomponent subunit A, C1QA

2 Images

Supplier Data

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.

PubMed

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.

Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.

Image from Phuan PW et al., J Biol Chem. 2012 Apr 20;287(17):13829-39. Epub 2012 Mar 5. Fig 7.; doi: 10.1074/jbc.M112.344325; April 20, 2012, The Journal of Biological Chemistry, 287, 13829-13839.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC, IHC-P, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human C1QA.

P02745

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-rabbit-igg-isotype-control-ab37406'>ab37406</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
FITC Anti-C1q antibody (ab4223) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-C1q FITC (ab4223) was first used in a scientific publication in 2003 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

See full target information C1QA

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013107 PubMed40294075

2025

Deposition of complement regulators on the surface of Plasmodium falciparum merozoites depends on the immune status of the host.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Rosaria Bassi,Bogdan Cristinoi,Frank Buitenwerf,Mark Bergholt Cuadrado,Kasper Haldrup Björnsson,Melanie Rose Walker,Frederica Dedo Partey,Andrew B Ward,Michael Fokuo Ofori,Lea Barfod

Transplant international : official journal of the European Society for Organ Transplantation 37:13407 PubMed39474587

2024

C1q Binding Ability for Prior Risk Assessment of Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Miwa,Kenta Iwasaki,Kenta Murotani,Manabu Okada,Takaharu Nagasaka,Yoshihiko Watarai,Asami Takeda,Masato Shizuku,Satoshi Ashimine,Kohei Ishiyama,Shoichi Maruyama,Takaaki Kobayashi

Nature communications 15:6966 PubMed39138196

2024

Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Dellière,Camille Chauvin,Sarah Sze Wah Wong,Markus Gressler,Valentina Possetti,Raffaella Parente,Thierry Fontaine,Thomas Krüger,Olaf Kniemeyer,Jagadeesh Bayry,Agostinho Carvalho,Axel A Brakhage,Antonio Inforzato,Jean-Paul Latgé,Vishukumar Aimanianda

Neural regeneration research 18:2413-2423 PubMed37282471

2023

A novel phenotype of B cells associated with enhanced phagocytic capability and chemotactic function after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Huaming Li,Chenhan Ling,Xiaotao Zhang,Jianan Lu,Weimin Luan,Jianmin Zhang,Ligen Shi

Proceedings of the National Academy of Sciences of the United States of America 120:e2217590120 PubMed37011197

2023

Subclass-switched anti-spike IgG3 oligoclonal cocktails strongly enhance Fc-mediated opsonization.

Applications

Unspecified application

Species

Unspecified reactive species

Arman Izadi,Arsema Hailu,Magdalena Godzwon,Sebastian Wrighton,Berit Olofsson,Tobias Schmidt,Anna Söderlund-Strand,Elizabeth Elder,Sofia Appelberg,Maria Valsjö,Olivia Larsson,Vidar Wendel-Hansen,Mats Ohlin,Wael Bahnan,Pontus Nordenfelt

Hepatology communications 6:2538-2550 PubMed35726350

2022

Spatial transcriptomics identifies enriched gene expression and cell types in human liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brian K Chung,Jonas Øgaard,Henrik Mikael Reims,Tom H Karlsen,Espen Melum

Cellular and molecular gastroenterology and hepatology 13:289-307 PubMed34536564

2021

C-Reactive Protein Protects Against Acetaminophen-Induced Liver Injury by Preventing Complement Overactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Yun Li,Zhao-Ming Tang,Zhe Wang,Jian-Min Lv,Xiao-Ling Liu,Yu-Lin Liang,Bin Cheng,Ning Gao,Shang-Rong Ji,Yi Wu

Infection and immunity 89:e0041221 PubMed34460286

2021

Anti-LPS IgA and IgG Can Inhibit Serum Killing of Pseudomonas aeruginosa in Patients with Cystic Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Amy Pham,Emma L Ledger,Carrie F Coggon,Ian R Henderson,David W Reid,Scott C Bell,Daniel J Smith,Timothy J Wells

Cell host & microbe 29:165-178.e8 PubMed33340449

2020

The immune landscape in tuberculosis reveals populations linked to disease and latency.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Esaulova,Shibali Das,Dhiraj Kumar Singh,Jose Alberto Choreño-Parra,Amanda Swain,Laura Arthur,Javier Rangel-Moreno,Mushtaq Ahmed,Bindu Singh,Ananya Gupta,Luis Alejandro Fernández-López,Maria de la Luz Garcia-Hernandez,Allison Bucsan,Chivonne Moodley,Smriti Mehra,Ethel García-Latorre,Joaquin Zuniga,Jeffrey Atkinson,Deepak Kaushal,Maxim N Artyomov,Shabaana A Khader

mAbs 11:826-836 PubMed30990348

2019

Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Michihiko Aoyama,Noritaka Hashii,Wataru Tsukimura,Kenji Osumi,Akira Harazono,Minoru Tada,Masato Kiyoshi,Akio Matsuda,Akiko Ishii-Watabe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-C1q antibody

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (15)

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