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AB4216

FITC Anti-C4c+C4b antibody

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(1 Publication)

Rabbit Polyclonal C4b antibody - conjugated to FITC. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-C4c+C4b antibody (AB4216)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-C4c+C4b antibody (AB4216)

Immunohistochemistry (Formalin fixed paraffin-embedded sections) analysis of human kidney stained labelling C4C Complement with ab4216.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with human C4, C4b and C4c but does not react with the C4d epitope.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C4c and C4b proteins also known as complement component 4c and complement component 4b play key roles in the complement system an essential part of immune defense. These proteins arise from the cleavage of complement component 4 (C4) during the classical and lectin pathways of complement activation. C4c and C4b are fragments with C4b possessing an estimated mass of around 195 kDa. They are expressed mainly in plasma cells as C4 and then are processed into C4b and C4c through enzymatic cleavage. The conversion allows them to participate in immune processes.
Biological function summary

Complement components C4c and C4b work to clear pathogens and immune complexes from the organism. C4b can attach to microbial surfaces promoting opsonization and facilitating phagocytosis. It forms part of the C3 convertase complex specifically in the C4b2a complex which plays a critical role in amplifying the complement response. Through their functions these components are involved in marking pathogens for elimination and helping to resolve infections.

Pathways

C4b and C4c serve significant roles in the classical and lectin pathways of the complement cascade. The C4b fragment in particular interacts with other proteins like C2 to form the C4b2a complex central to the activation of C3 convertase. This interaction is critical in promoting the cleavage of C3 into C3a and C3b further propagating the complement activation. By engaging in these pathways C4b and C4c facilitate innate immune responses.

The components C4c and C4b relate to conditions such as systemic lupus erythematosus (SLE) and hereditary angioedema. In SLE improper clearance of immune complexes and apoptotic cells can result from deficient C4b activity potentially linking to the disease's characteristic autoimmunity. Hereditary angioedema involves imbalances in the complement system where C4 levels can serve as markers in the diagnostic process. Additionally C4 deficiency or dysfunction may connect these disorders to regulatory proteins like C1 inhibitor emphasizing the complementary system's impact on disease expression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
See full target information C4B

Additional targets

C4B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virulence 9:1138-1149 PubMed30067453

2018

Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Ispasanie,Francesca Micoli,Araceli Lamelas,Dominique Keller,Francesco Berti,Riccardo De Riccio,Roberta Di Benedettoi,Simona Rondini,Gerd Pluschke
View all publications
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Complementary Products

Assay Kits

AB277717

Human C4b ELISA Kit (Complement C4-B)

Recombinant|SimpleStep

ELISA - Human C4b ELISA Kit (Complement C4-B) (AB277717)

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Primary Antibodies

AB87424

Anti-C4d antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody (AB87424)

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Primary Antibodies

AB4223

FITC Anti-C1q antibody

Immunocytochemistry - FITC Anti-C1q antibody (AB4223)

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Primary Antibodies

AB134943

Anti-C1s antibody [EPR9066(B)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (AB134943)

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Primary Antibodies

AB275931

Anti-C5 / C5b antibody [EPR23940-3]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931)

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  • 0

Product promise

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