AB11466

FITC Anti-CCR5 antibody [HEK/1/85a]

Name: Anti-CCR5 FITC antibody [HEK/1/85a] (ab11466) Rat monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB11466
Price: 270 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(7 Publications)

Rat Monoclonal CCR5 antibody - conjugated to FITC. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

CD195, CMKBR5, CCR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

HEK/1/85a

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-Fr, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purification by affinity chromatography.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR5 also known as the C-C chemokine receptor type 5 is a protein that functions as a receptor on the surface of cells predominantly immune cells like T-cells macrophages and dendritic cells. The approximate molecular weight of CCR5 is 40 kDa. This protein belongs to the G-protein-coupled receptor family and it mainly binds to β-chemokines. CCR5 is abundantly expressed in immune tissues such as the spleen thymus and lymph nodes. Researchers often use assays like the CCR5 ELISA kit to detect or quantify its presence in various samples.

Biological function summary

CCR5 acts as a receptor for various chemokines specifically CCL3 CCL4 and CCL5 which are involved in leukocyte trafficking and immune response modulation. It plays an important role in the migration of immune cells to sites of inflammation or injury. This protein does not function in isolation; it sometimes forms part of larger complexes with other cell surface proteins to mediate responses to external signals. For example anti-CCR5 antibodies like 12D1 can modulate its activity and influence immune cell behavior.

Pathways

CCR5 is intricately involved in chemokine signaling pathways and inflammatory response pathways. Through these pathways it interacts with proteins such as CXCR4 and CCR1. CCR5 and CXCR4 another chemokine receptor are well-documented for their role in HIV entry into host cells. These pathways are critical for orchestrating immune cell movement and activating signaling cascades that promote immune defense mechanisms.

CCR5 has a significant association with HIV infection and is a known co-receptor facilitating the virus's entry into immune cells. The presence of mutations like the Δ32 allele in the CCR5 gene provides resistance against HIV infection highlighting its importance in disease dynamics. CCR5 also plays a role in inflammatory diseases such as rheumatoid arthritis where it contributes to the migration of immune cells into joints exacerbating inflammation. In these contexts CCR5 interacts with other inflammatory mediators offering potential therapeutic targets for drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed : 30713770).. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.

See full target information CCR5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37903056

2023

CCR1 mediates Müller cell activation and photoreceptor cell death in macular and retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Elbaz-Hayoun,Batya Rinsky,Shira Hagbi-Levi,Michelle Grunin,Itay Chowers

Journal of translational medicine 21:196 PubMed36918921

2023

CCL5/CCR5-mediated peripheral inflammation exacerbates blood‒brain barrier disruption after intracerebral hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Lin,Ya Xu,Peiwen Guo,Yù-Jié Chen,Jiru Zhou,Min Xia,Binbin Tan,Xin Liu,Hua Feng,Yujie Chen

Acta pharmaceutica Sinica. B 12:228-245 PubMed35127382

2022

Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Li,Yao Zhang,Moting Liu,Chen Fan,Chunlan Feng,Qiukai Lu,Caigui Xiang,Huimin Lu,Xiaoqian Yang,Bing Wu,Duowu Zou,Wei Tang

PLoS pathogens 16:e1008359 PubMed32084246

2020

Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Charles A Coomer,Irene Carlon-Andres,Maro Iliopoulou,Michael L Dustin,Ewoud B Compeer,Alex A Compton,Sergi Padilla-Parra

British journal of pharmacology 176:2209-2226 PubMed30883697

2019

Inhibition of phosphodiesterase-4 attenuates murine ulcerative colitis through interference with mucosal immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Li,Chen Fan,Chunlan Feng,Yanwei Wu,Huimin Lu,Peilan He,Xiaoqian Yang,Fenghua Zhu,Qing Qi,Yuanzhuo Gao,Jianping Zuo,Wei Tang

Scientific reports 8:1115 PubMed29348435

2018

Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav P Mukherjee,Olesja Bondarenko,Pekka Kohonen,Fernando T Andón,Táňa Brzicová,Isabel Gessner,Sanjay Mathur,Massimo Bottini,Paolo Calligari,Lorenzo Stella,Elena Kisin,Anna Shvedova,Reija Autio,Heli Salminen-Mankonen,Riitta Lahesmaa,Bengt Fadeel

Nature communications 8:2226 PubMed29263385

2017

The HIV co-receptor CCR5 regulates osteoclast function.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Won Lee,Akiyoshi Hoshino,Kazuki Inoue,Takashi Saitou,Shunsuke Uehara,Yasuhiro Kobayashi,Satoshi Ueha,Kouji Matsushima,Akira Yamaguchi,Yuuki Imai,Tadahiro Iimura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CCR5 antibody [HEK/1/85a]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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