JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB95670

FITC Anti-CCR9 antibody [CW-1.2]

4

(5 Reviews)

|

(1 Publication)

Mouse Monoclonal CCR9 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Ccr9 protein.

View Alternative Names

CDw199, Cmkbr10, Ccr9, C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10

2 Images
Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (AB95670)
  • Flow Cyt

AbReview29145****

Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (AB95670)

ab95670 staining CCR9 in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.

This image is courtesy of an anonymous Abreview

Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (AB95670)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CCR9 antibody [CW-1.2] (AB95670)

Flow cytometry staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a kappa Isotype Control FITC (open histogram) or 0.125 µg of ab95670 (filled histogram). Cells in the lymphocyte gate were used for analysis and gated on either CD4+ (left) or CD8+ (right) events.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CW-1.2

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Ccr9 protein. The exact immunogen used to generate this antibody is proprietary information.

Q9WUT7

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A final volume of 100 μL is recommended for staining the cell sample.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 2.68% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR9 also known as CD199 is a chemokine receptor known for its role in guiding cell traffic. It is a G-protein-coupled receptor with a mass of approximately 43 kDa. CCR9 is mainly expressed on the surface of thymocytes T lymphocytes and some subsets of dendritic cells. Its presence is especially noted in the small intestine correlated with its role in the migration and function of immune cells in the gut. This receptor is important for the homing of these cells to the intestinal mucosa engaging specifically with its ligand CCL25.
Biological function summary

CCR9 plays a significant role in the immune system particularly in the gut. It is part of chemokine receptor-ligand complexes that are integral to leukocyte trafficking. By interacting with CCL25 CCR9 facilitates the directed movement of immune cells across intestinal tissue impacting the immune surveillance and response within this environment. The receptor's expression pattern also suggests involvement in thymocyte development supporting the immune system's ability to generate a diverse repertoire of T cells.

Pathways

CCR9 is an important component of the chemokine signaling pathway. It is intricately connected to G-protein signaling events triggering downstream effects that drive cell migration and adhesion. This pathway is important for the function and maintenance of mucosal immunity linking CCR9 with CCR7 and its role in guiding T cell migration to specific tissue sites. These pathways collectively support immune functionality and homeostasis underlining CCR9's significance in tissue-specific immunity.

CCR9 has associations with inflammatory disorders like Crohn's disease and ulcerative colitis. Inflammatory bowel disease (IBD) is marked by aberrant immune cell movement and function areas where CCR9 is particularly influential. The receptor's interaction with CCL25 is pivotal in these pathological conditions often being a therapeutic target to modulate immune cell trafficking in disease management. Additionally the altered expression of CCR9 may contribute to the progression of specific T-cell leukemias further emphasizing its clinical relevance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
See full target information Ccr9
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 36:73-81 PubMed16342233

2005

Complex regulation of CCR9 at multiple discrete stages of T cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-André Wurbel,Bernard Malissen,James J Campbell
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB52590

Anti-CCR9 antibody [7E7]

Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (AB52590)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB256268

Alexa Fluor® 488 Anti-CD1a antibody [NA1/34]

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD1a antibody [NA1/34] (AB256268)

  • 0
  • 0
Primary Antibodies

AB203829

Anti-ZEB1 antibody [EPR17375]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] (AB203829)

  • 3
  • 3
Primary Antibodies

AB8396

Anti-BAFF antibody

Western blot - Anti-BAFF antibody (AB8396)

  • 0
  • 0
Primary Antibodies

AB313468

Anti-DR3/LARD Antibody [RP23040174]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DR3/LARD Antibody [RP23040174] (AB313468)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com