AB28096

FITC Anti-CD10 antibody [MEM-78]

Name: FITC Anti-CD10 antibody [MEM-78] (ab28096) | Abcam
Brand: Abcam Limited
SKU: AB28096
Price: 95 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD10 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MME protein.

View Alternative Names

CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

2 Images

Flow Cytometry - FITC Anti-CD10 antibody [MEM-78] (AB28096)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD10 antibody [MEM-78] (AB28096)

Separation of human neutrophil granulocytes (red-filled) from 10 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28096 (20 ul reagent / 100 ul of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD10 antibody [MEM-78] (AB28096)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28096 (20 ul reagent / 100 ul of peripheral whole blood).

660 APC

APC Anti-CD-10 antibody [MEM-78]
785 APC/Cy7®

APC/Cy7® Anti-CD10 antibody [MEM-78]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-CD10 antibody [MEM-78]
578 PE

PE Anti-CD-10 antibody [MEM-78]
667 PE/Cy5®

PE/Cy5® Anti-CD10 antibody [MEM-78]
778 PE/Cy7®

PE/Cy7® Anti-CD10 antibody [MEM-78]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD10 antibody [MEM-78]
675 PerCP

PerCP Anti-CD10 antibody [MEM-78]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD10 antibody [MEM-78]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-78

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MME protein. The exact immunogen used to generate this antibody is proprietary information.

P08473

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" } } }

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Product details

The antibody is free of unconjugated FITC.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.

Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).

See full target information MME

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Folia biologica 34:23-34 PubMed2968928

1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I Hilgert

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD10 antibody [MEM-78]

Flow Cytometry - FITC Anti-CD10 antibody [MEM-78] (AB28096)

Flow Cytometry - FITC Anti-CD10 antibody [MEM-78] (AB28096)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise