AB53318

FITC Anti-CD105 antibody [MEM-229]

Name: Anti-CD105 FITC antibody [MEM-229] (ab53318) Mouse monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB53318
Price: 270 USD
Availability: InStock

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(26 Publications)

Mouse Monoclonal CD105 antibody - conjugated to FITC. Suitable for IHC-Fr and reacts with Pig samples. Cited in 26 publications. Immunogen corresponding to Recombinant Virus within Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

1 Images

Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (AB53318)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (AB53318)

ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-229

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Pig

Applications

IHC-Fr

applications

Immunogen

Recombinant Virus within Human ENG. The exact immunogen used to generate this antibody is proprietary information.

P17813

Specificity

The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B
lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "<p>Fix with acetone.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).

See full target information ENG

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1478757 PubMed39568509

2024

Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Khaveh,René Buschow,Julia Metzger

Animals : an open access journal from MDPI 13: PubMed37627392

2023

Isolation of Vascular Wall Mesenchymal Stem Cells from the Thoracic Aorta of Adult Göttingen Minipigs: A New Protocol for the Simultaneous Endothelial Cell Collection.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Bernardini,Debora La Mantia,Roberta Salaroli,Domenico Ventrella,Alberto Elmi,Augusta Zannoni,Monica Forni

Advanced healthcare materials 12:e2301180 PubMed37463568

2023

Selective Extracellular Matrix Guided Mesenchymal Stem Cell Self-Aggregate Engineering for Replication of Meniscal Zonal Tissue Gradient in a Porcine Meniscectomy Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Noh,Yong Jun Jin,Dong Il Shin,Hyeon Jae Kwon,Hee-Woong Yun,Kyu Min Kim,Jae-Young Park,Jun Young Chung,Do Young Park

Basic and clinical neuroscience 13:625-636 PubMed37313021

2022

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Ardeshir Moayeri,Rafieh Alizadeh,Hatef Ghasemi Hamidabadi,Maryam Nazm Bojnordi,Ali Niapour,Azim Hedayatpour,Marzieh Darvishi,Fatemeh Heidari,Maryam Soleimani,Leila Elyasi

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:2646-2655 PubMed35112388

2022

Biomolecules-releasing click chemistry-based bioadhesives for repairing acetabular labrum tears.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxin Li,Cynthia M Co,Samira Izuagbe,Yi Hong,Jun Liao,Joseph Borrelli,Liping Tang

Journal of orthopaedic surgery and research 16:43 PubMed33430899

2021

Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwon Oh,Yeon Sung Son,Wan Hee Kim,Oh-Kyeong Kwon,Byung-Jae Kang

Clinical, cosmetic and investigational dermatology 13:957-971 PubMed33364805

2020

Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxiu Cao,Bei Chen,Xian Zhang,Hongyun Chen

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

Molecular therapy. Nucleic acids 19:1237-1249 PubMed32069774

2020

MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Haiyan Ding,Min Wei,Wenhui Zha,Shuang Guan,Ning Liu,Yang Li,Yuan Tan,Yan Wang,Fuju Wu

World journal of gastroenterology 25:4892-4903 PubMed31543681

2019

effect of vascular wall stromal cells secretome on enteric ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Dothel,Chiara Bernardini,Augusta Zannoni,Maria Rosaria Spirito,Roberta Salaroli,Maria Laura Bacci,Monica Forni,Fabrizio De Ponti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD105 antibody [MEM-229]

Immunohistochemistry (Frozen sections) - FITC Anti-CD105 antibody [MEM-229] (AB53318)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

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