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AB95684

FITC Anti-CD116 antibody [4H1]

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(2 Publications)

Mouse Monoclonal CD116 antibody - conjugated to FITC. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CSF2RA protein.

View Alternative Names

CD116, CSF2R, CSF2RY, CSF2RA, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116

2 Images
Immunocytochemistry/ Immunofluorescence - FITC Anti-CD116 antibody [4H1] (AB95684)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD116 antibody [4H1] (AB95684)

Immunofluorescence analysis of CD116 was performed using 70% confluent log phase THP-1 cells treated with 10 ng of GM-CSF for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD116, FITC, using ab95684 at 1 : 100 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin (1 : 300). Panel d represents the merged image showing membrane localization. Panel e shows untreated cells with less membrane signal. Panel f represents the isotype control. The images were captured at 60X magnification.

Flow Cytometry - FITC Anti-CD116 antibody [4H1] (AB95684)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD116 antibody [4H1] (AB95684)

Flow cytometry staining of normal Human peripheral blood cells with Mouse IgG1 - Isotype Control FITC (open histogram) or 20μl ab95684 (filled histogram). Cells in the monocyte gate were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4H1

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Cell preparation containing CSF2RA protein. The exact immunogen used to generate this antibody is proprietary information.

P15509

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-kappa-mopc-21-isotype-control-ab106163'>ab106163</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).
Biological function summary

The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.

Pathways

CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.

Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
See full target information CSF2RA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0265725 PubMed35316296

2022

Pancreatic Ductal Adenocarcinoma (PDAC) circulating tumor cells influence myeloid cell differentiation to support their survival and immunoresistance in portal vein circulation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Pablo Arnoletti,Joseph Reza,Armando Rosales,Alberto Monreal,Na'im Fanaian,Suzanne Whisner,Milan Srivastava,Julia Rivera-Otero,Gongxin Yu,Otto Phanstiel Iv,Deborah A Altomare,Quang Tran,Sally A Litherland

Immunity, inflammation and disease 9:144-156 PubMed33156578

2020

Secreted TRAIL gene-modified adipose-derived stem cells exhibited potent tumor-suppressive effect in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Liu,Shaojie Li,Tiexiang Ma,Jian Zeng,Xin Zhou,Huanyu Li,Min Tang,Xiang Liu,Feng Li,Bin Jiang,Ming Zhao,Ying Chen
View all publications
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Product promise

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