JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB34444

FITC Anti-CD11b antibody [2F4/11]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD11b antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Pig samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD11b protein.
3 Images
Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse IgG1 isotype control. Figure B. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse anti porcine CD11R3 (ab34444).

All experiments performed on red cell lysed porcine blood gated on lymphocytes (10ul neat to label 1x106 cells in 100ul).

Data acquired on the ZE5 Cell Analyzer.

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse IgG1 isotype control. Figure B. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse anti porcine CD11R3 (ab34444).

All experiments performed on red cell lysed porcine blood gated on mononuclear cells (10ul neat to label 1x106 cells in 100ul) .

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. FITC conjugated mouse anti porcine CD11R3 (ab34444) and APC conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti porcine CD11R3 (ab34444) and APC conjugated mouse anti porcine CD31.

All experiments performed on red cell lysed porcine blood gated on mononuclear cells (10ul neat to label 1x106 cells in 100ul).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F4/11

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Pig

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab34444 is expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.

style
left-column

Alternative Products

Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
  • 1
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB52478

Anti-CD11b antibody [EP1345Y] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478)

  • 5
  • 5
Primary Antibodies

AB187537

Anti-CD11b antibody [EP1345Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - BSA and Azide free (AB187537)

  • 4
  • 1
Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB198934

Alexa Fluor® 647 Anti-Fibronectin antibody [F1]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (AB198934)

  • 0
  • 0
Primary Antibodies

AB218714

Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] (AB218714)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB233046

Alexa Fluor® 647 Anti-CD11c antibody [N418]

Flow Cytometry - Alexa Fluor® 647 Anti-CD11c antibody [N418] (AB233046)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Neat - 1/10. Use 10ul of the suggested working dilution to 1x10^6 cells in 100ul. <a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

ab34444 is reported to block phagocytosis of complement-opsonised zymosan particles by polymononuclear granulocytes and alveolar macrophages.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information CD11b
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in plant science 11:440 PubMed32328082

2020

Removal of N-Terminal Fusion Domains From Recombinant Target Proteins Produced in .

Applications

Unspecified application

Species

Unspecified reactive species

Md Reyazul Islam,Seoyoung Choi,Thangarasu Muthamilselvan,Kunyoo Shin,Inhwan Hwang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com