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AB24874

FITC Anti-CD11b antibody [M1/70]

4

(3 Reviews)

|

(25 Publications)

Rat Monoclonal CD11b antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 25 publications. Immunogen corresponding to Cell preparation containing Itgam protein.

View Alternative Names

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

1 Images
Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (AB24874)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (AB24874)

Flow Cytometry analysis of Mouse C57BL/6 bone marrow cells labeling CD11b with ab24874 at 0.5μg (solid line), Rat IgG2b (FITC) isotype control (dashed line) at 0.5μg concentration.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • Unconjugated

    Anti-CD11b antibody [M1/70]

  • Carrier free

    Anti-CD11b antibody [M1/70] - BSA and Azide free

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11b antibody [M1/70]

  • redFluor™ 710

    redFluor™ 710 Anti-CD11b antibody [M1/70]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [M1/70]

  • Biotin

    Biotin Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11b antibody [M1/70]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

Specificity

Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1581878 PubMed40539039

2025

Identification of the microglia-associated signature in experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wu,Jianhong Wang,Bo Chen,Yuxue Guo,Ping Gan,Yanbing Han

Glia 73:1272-1287 PubMed39988890

2025

Generation of an Inducible Destabilized-Domain Cre Mouse Line to Target Disease Associated Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Caden M Henningfield,Minh Ngo,Kaitlin M Murray,Nellie E Kwang,Kate I Tsourmas,Jonathan Neumann,Zachary A Pashkutz,Shimako Kawauchi,Vivek Swarup,Thomas E Lane,Grant R MacGregor,Kim N Green

Acta pharmaceutica Sinica. B 14:3983-4000 PubMed39309491

2024

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Li,Riqing Wei,Chenxin Liu,Keying Zhang,Sixiao He,Zhifeng Liu,Junhao Huang,Youyong Tang,Qiyuan An,Ligen Lin,Lishe Gan,Liying Zhao,Xiaoming Zou,Fudi Wang,Yuan Ping,Qiang Ma

Cellular & molecular biology letters 29:100 PubMed38977961

2024

New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Lu,Xiaojian Shi,Qiang Fu,Yaguang Han,Lei Zhu,Zhibin Zhou,Yongchuan Li,Nan Lu

Journal of neuroinflammation 21:157 PubMed38879499

2024

Cystatin F attenuates neuroinflammation and demyelination following murine coronavirus infection of the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Amber R Syage,Collin Pachow,Kaitlin M Murray,Caden Henningfield,Kellie Fernandez,Annie Du,Yuting Cheng,Gema Olivarria,Shimako Kawauchi,Grant R MacGregor,Kim N Green,Thomas E Lane

Stem cells international 2023:2759679 PubMed37576407

2023

miR-4780 Derived from N2-Like Neutrophil Exosome Aggravates Epithelial-Mesenchymal Transition and Angiogenesis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Yuqiang Shan,Sixin Zheng,Jiangtao Li,Peng Cui

International journal of biological sciences 19:1123-1145 PubMed36923940

2023

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian Zhu

Frontiers in microbiology 13:958132 PubMed36212814

2022

Cocktail of isobavachalcone and curcumin enhance eradication of biofilm from orthopedic implants by gentamicin and alleviate inflammatory osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Hao Hu,Fangli Huang,Zemin Ling,Bolin Chen,Bizhi Tan,Tingxuan Wang,Xiao Liu,Chun Liu,Xuenong Zou

Frontiers in chemistry 10:955995 PubMed36034656

2022

Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Shang,Juntao Sun,Xin Wu,Qinghai Wang

Frontiers in endocrinology 13:871548 PubMed35634492

2022

ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ani Chi,Bicheng Yang,Xiaohui Cao,Zhenqing Wang,Hanchao Liu,Hao Dai,Chunhua Deng,Min Zhang
View all publications
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