AB22540

FITC Anti-CD11c antibody [BU15]

Name: Anti-CD11c FITC antibody [BU15] (ab22540) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB22540

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(4 Publications)

Mouse Monoclonal CD11c antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Cynomolgus monkey, Human, Dog samples. Cited in 4 publications.

View Alternative Names

CD11c, CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BU15

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Dog, Human, Cynomolgus monkey

Applications

Flow Cyt

applications

Specificity

This antibody recognises the integrin alphax subunit, a 150kD glycoprotein expressed by macrophages, monocytes, granulocytes and NK cells. The antigen is also expressed by hairy cell leukaemia cells. Ligands for CD11c are ICAM 1, iC3b, and fibrinogen. The molecule has a role in mediating adherence of granulocytes and monocytes to endothelium and fibrinogen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of working dilution for 106 cells." }, "Cynomolgus monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of working dilution for 106 cells." }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of working dilution for 106 cells." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.

Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

See full target information ITGAX

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

mBio 11: PubMed32665270

2020

Convergent Evolution of HLA-C Downmodulation in HIV-1 and HIV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Hopfensperger,Jonathan Richard,Christina M Stürzel,Frederic Bibollet-Ruche,Richard Apps,Marie Leoz,Jean-Christophe Plantier,Beatrice H Hahn,Andrés Finzi,Frank Kirchhoff,Daniel Sauter

Cell reports 27:2092-2104.e10 PubMed31091448

2019

Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Braun,Dominik Hotter,Lennart Koepke,Fabian Zech,Rüdiger Groß,Konstantin M J Sparrer,Janis A Müller,Christian K Pfaller,Elena Heusinger,Rebecka Wombacher,Kathrin Sutter,Ulf Dittmer,Michael Winkler,Graham Simmons,Martin R Jakobsen,Karl-Klaus Conzelmann,Stefan Pöhlmann,Jan Münch,Oliver T Fackler,Frank Kirchhoff,Daniel Sauter

eLife 8: PubMed30717826

2019

HIV-1 Vpu is a potent transcriptional suppressor of NF-κB-elicited antiviral immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Langer,Christian Hammer,Kristina Hopfensperger,Lukas Klein,Dominik Hotter,Paul D De Jesus,Kristina M Herbert,Lars Pache,Nikaïa Smith,Johannes A van der Merwe,Sumit K Chanda,Jacques Fellay,Frank Kirchhoff,Daniel Sauter

Blood 97:2932-40 PubMed11342414

2001

Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.

Applications

Unspecified application

Species

Unspecified reactive species

R Rezzonico,V Imbert,R Chicheportiche,J M Dayer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD11c antibody [BU15]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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