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AB33486

FITC Anti-CD13 antibody [R3-63]

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(2 Publications)

Rat Monoclonal CD13 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD13, Lap-1, Lap1, Anpep, Aminopeptidase N, AP-N, mAPN, Alanyl aminopeptidase, Aminopeptidase M, Membrane protein p161, Microsomal aminopeptidase, AP-M

  • Unconjugated

    Anti-CD13 antibody [R3-63]

  • 578 PE

    PE Anti-CD13 antibody [R3-63]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R3-63

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

Recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13.

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Alternative Products

Primary Antibodies

AB197630

Biotin Anti-CD13 antibody [EPR4058]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD13 antibody [EPR4058] (AB197630)

  • 0
  • 0
Primary Antibodies

AB108382

Anti-CD13 antibody [EPR4059]

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  • 5
  • 3
Primary Antibodies

AB108310

Anti-CD13 antibody [EPR4058]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (AB108310)

  • 4
  • 10
Primary Antibodies

AB196576

Anti-CD13 antibody [EPR4059] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] - BSA and Azide free (AB196576)

  • 1
  • 1
Primary Antibodies

AB227111

Anti-CD13 antibody [EPR4058] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] - Low endotoxin, Azide free (AB227111)

  • 0
  • 0
Primary Antibodies

AB243926

Anti-CD13 antibody [SP182] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [SP182] - BSA and Azide free (AB243926)

  • 0
  • 0
Primary Antibodies

AB245741

Anti-CD13 antibody [SP187] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [SP187] - BSA and Azide free (AB245741)

  • 0
  • 0

Complementary Products

Assay Kits

AB239427

Human CD13 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Assay Kits

AB239427

Human CD13 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

  • 0
  • 0
Primary Antibodies

AB226

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (AB226)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. <a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Clone R3-63 has been reported to block mouse APN enzyme activity.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.
Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines (By similarity). May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells (PubMed : 8691132). May have a role in angiogenesis and promote cholesterol crystallization (By similarity). May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (PubMed : 22677001).
See full target information Anpep
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:1182-1194 PubMed37215441

2023

Differentiation of imatinib -resistant chronic myeloid leukemia cells with BCR-ABL-T315I mutation induced by Jiyuan Oridonin A.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Xu,Ziting Wang,Lei Zhang,Congying Gao,Fahui Li,Xueming Li,Yu Ke,Hong-Min Liu,Zhenbo Hu,Liuya Wei,Zhe-Sheng Chen

BMC genomics 18:946 PubMed29202695

2017

Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development.

Applications

Unspecified application

Species

Unspecified reactive species

Xianbin Su,Yi Shi,Xin Zou,Zhao-Ning Lu,Gangcai Xie,Jean Y H Yang,Chong-Chao Wu,Xiao-Fang Cui,Kun-Yan He,Qing Luo,Yu-Lan Qu,Na Wang,Lan Wang,Ze-Guang Han
View all publications
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Product promise

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