AB28061

FITC Anti-CD14 antibody [MEM-15]

Name: FITC Anti-CD14 antibody [MEM-15] (ab28061) | Abcam
Brand: Abcam Limited
SKU: AB28061
Price: 95 USD
Availability: InStock
Rating: 5 (6 reviews)

(6 Reviews)

(13 Publications)

Mouse Monoclonal CD14 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

2 Images

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.

Flow Cyt

AbReview15883****

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

ab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.

This image is courtesy of an anonymous Abreview

660 APC

APC Anti-CD14 antibody [MEM-15]
785 APC/Cy7®

APC/Cy7® Anti-CD14 antibody [MEM-15]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-CD14 antibody [MEM-15]
Biotin

Biotin Anti-CD14 antibody [MEM-15]
667 PE/Cy5®

PE/Cy5® Anti-CD14 antibody [MEM-15]
778 PE/Cy7®

PE/Cy7® Anti-CD14 antibody [MEM-15]
675 PerCP

PerCP Anti-CD14 antibody [MEM-15]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD14 antibody [MEM-15]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-15

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human CD14.

P08571

Specificity

Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood.</p>" } } }

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Product details

The antibody is free of unconjugated FITC.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e23059 PubMed38149183

2023

In vitro immunomodulatory effect of solid versus porous phosphate-based glass microspheres using macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Soumya Sheela,Fatma Mousa AlGhalban,Ifty Ahmed,Ensanya A Abou Neel

Annals of biomedical engineering 52:48-56 PubMed37989902

2023

Harnessing the Electrochemical Effects of Electroporation-Based Therapies to Enhance Anti-tumor Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Zaid S Salameh,Kenneth N Aycock,Nastaran Alinezhadbalalami,Khan Mohammad Imran,Iain H McKillop,Irving C Allen,Rafael V Davalos

Journal of lipid research 64:100380 PubMed37094639

2023

Effect of type 2 diabetes on the inducible degrader of LDL receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Sum Lam,David Tak Wai Lui,Sammy Wing Ming Shiu,Ying Wong,Kathryn Choon Beng Tan

Stem cells international 2022:2164057 PubMed36311041

2022

miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baodong Wang,Na Xu,Li Cao,Xiaojun Yu,Shanxi Wang,Qikun Liu,Yinguang Wang,Haoran Xu,Yang Cao

Endocrine connections 11: PubMed35560019

2022

Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Lok-Yi Chan,Sammy Wing-Ming Shiu,Ching-Lung Cheung,Anskar Yu-Hung Leung,Kathryn Choon-Beng Tan

Cell death discovery 7:224 PubMed34455417

2021

Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shui Ma,Ji-Bin Liu,Lan Lin,Hui Zhang,Jian-Jun Wu,Yi Shi,Cheng-You Jia,Dan-Dan Zhang,Fei Yu,Hui-Min Wang,Yu-Zhen Yin,Xiao-Hui Jiang,Pei-Yao Wang,Lin-Lin Tian,Ping-Sheng Cao,Xu-Ming Wu,Hai-Min Lu,Li-Peng Gu,Jia-Jia Zhang,Gu-Jun Cong,Pei Luo,Xiao-Ming Zhong,Bo Cai,Min-Xin Shi,Su-Qing Zhang,Liu Li,Wen-Jie Zhang,Yu Liu,Zhi-Zhen Li,Ting-Miao Wu,Zhi-Jun Wu,Gao-Ren Wang,Zhong-Wei Lv,Chang-Chun Ling,Kai-Jian Chu,Da Fu

Journal of cellular and molecular medicine 25:1867-1883 PubMed33417281

2021

Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Meng,Baofang Zhang,Yingming Zhang,Shuyan Wang,Xiaohui Zhu

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

Stem cell research & therapy 8:246 PubMed29096715

2017

Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Shi,JingWei Gao,Yao Jiang,Baolan Sun,Wei Lu,Min Su,Yunzhao Xu,Xiaoqing Yang,Yuquan Zhang

Oncology letters 14:1766-1774 PubMed28789407

2017

Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Gonda,Masahiko Shibata,Tohru Ohtake,Yoshiko Matsumoto,Kazunoshin Tachibana,Noriko Abe,Hitoshi Ohto,Kenichi Sakurai,Seiichi Takenoshita

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD14 antibody [MEM-15]

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise