Skip to main content

Mouse Monoclonal CD146 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing MCAM protein.

Be the first to review this product! Submit a review

Images

Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (AB78451), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 1.5% BSA, 1.45% Sodium chloride, 0.0536% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing MCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P43121

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
2 µg for 106 Cells
Notes

Methanol fixed cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Alternative names

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Recommended products

Mouse Monoclonal CD146 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing MCAM protein.

Key facts

Isotype
IgG1
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing MCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P43121
Clone number
P1H12
Purification technique
Affinity purification Immunogen
Specificity

ab78451 reacts specifically with CD146.
In blood and marrow ab78451 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples.
ab78451 positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, EBV-transformed B cell lines, normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with ab78451 are also positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.

Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

Associated diseases and disorders

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451), expandable thumbnail

    Flow Cytometry - FITC Anti-CD146 antibody [P1H12] (ab78451)

    Overlay histogram showing MCF-7 cells stained with ab78451 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78451, 2µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com