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Mouse Monoclonal CD19 antibody - conjugated to FITC. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

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Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: 0.5% BSA

Form

Liquid

Clonality

Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytFlow Cyt (Intra)
Human
Expected
Expected

Expected
Expected

Species

Human

Dilution info

-

Notes

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10μl of CD19 (FITC) is sufficient for labelling of 1x106 cells.

Expected
Expected

Species

Human

Dilution info

-

Notes

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target data

Function

Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701).

Alternative names

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Mouse Monoclonal CD19 antibody - conjugated to FITC. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

Alternative names

Key facts

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Clone number

CB19

Light chain type

unknown

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

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