Mouse Monoclonal CD20 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing MS4A1 protein.
Images
![Flow Cytometry - FITC Anti-CD20 antibody [2H7] (AB51792), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd20-antibody-2h7-ab51792--flow-cytometry-img26098.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-CD20 antibody [2H7] (AB51792)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11836
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µL for 105-108 Cells | Notes The antibody has been diluted for use at 5 μl (0.25 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl. |
Associated Products
Select an associated product type
6 products for Alternative Version
6 products for Alternative Product
Target data
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Alternative names
CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
Recommended products
Mouse Monoclonal CD20 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing MS4A1 protein.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11836
- Clone number
- 2H7
- Purification technique
- Affinity purification
- Epitope
- The epitope recognised by this antibody has been mapped to the following sequence which is found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Do Not Freeze, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - FITC Anti-CD20 antibody [2H7] (ab51792), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd20-antibody-2h7-ab51792--flow-cytometry-img26098.png "Flow Cytometry - FITC Anti-CD20 antibody [2H7] (ab51792)")
Flow Cytometry - FITC Anti-CD20 antibody [2H7] (ab51792)
Flow cytometry analysis showing human peripheral blood lymphocytes stained with ab51792 (solid line) and isotype control antibody was FITC Mouse IgG2b (dashed line).
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