FITC Anti-CD20 antibody [EP459Y]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD20 antibody [EP459Y] (AB322915)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab322915 (right) or Rabbit IgG [EPR25A] FITC Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab322915 or Rabbit IgG [EPR25A] FITC Isotype Control (1x 10⁶ in 100 μl at 0.0016 μg/ml (1/312500)) for 30 min at 4°C. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable lymphocytes.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD20 antibody [EP459Y] (AB322915)

Flow cytometry overlay histogram showing left Ramos positive cells and right negative HEK293 stained with ab322304 (red line). The cells were incubated in 1x PBS containing μg/ml human IgG and 10% normal goat serum followed by the antibody (ab322915) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG [EPR25A] FITC used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.