Armenian hamster Monoclonal CD28 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Mouse samples. Cited in 2 publications.
Images
![Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (AB24844), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd28-antibody-pv-1-ab24844--flow-cytometry-img6858.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (AB24844)")
Publications
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- International immunology 7:891-9041995CD28 ligands CD80 (B7-1) and CD86 (B7-2) induce long-term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesB L Levine,Y Ueda,N Craighead,M L Huang,C H JunePubMed 7577797
- Journal of immunology (Baltimore, Md. : 1950) 154:985-971995Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Abe,P Vandenberghe,N Craighead,D S Smoot,K P Lee,C H JunePubMed 7822814
Key facts
- Isotype
IgG
- Host species
Armenian hamster
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg for 106 Cells | Notes ab18473 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info 3 µg for 106 Cells | Notes ab18473 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
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6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Alternative names
T-cell-specific surface glycoprotein CD28, TP44, CD28
Recommended products
Armenian hamster Monoclonal CD28 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Armenian hamster
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Clone number
PV-1
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd28-antibody-pv-1-ab24844--flow-cytometry-img6858.jpg "Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)")
Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (ab24844)
ab24844 at a concentration of 3 μg/106 cells staining human A 20 (B cell lymphoma cells) and EL-4 cells by flow cytometry. A 50:50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with ab24844. Large cells were gated and analyzed by flow cytometry.
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