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AB239226

FITC Anti-CD3 antibody [145-2C11]

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(2 Publications)

Hamster Monoclonal CD3d antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd3e protein.

View Alternative Names

CD3d, T3d, Cd3d, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

3 Images
Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)

Flow Cytometry analysis of murine splenocytes labeling CD3 with ab239226 (green). Surface staining.

Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)

Flow cytometry (surface staining) of murine splenocytes stained with 1μg/ml ab239226

Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD3 antibody [145-2C11] (AB239226)

Flow cytometry surface staining analysis of murine splenocytes with CD3 positive cells stained with 1μg/ml ab239226 (red) and CD3 negative cells (black)

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    APC Anti-CD3 epsilon antibody [145-2C11]

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    PerCP Anti-CD3 epsilon antibody [145-2C11]

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Key facts

Host species

Hamster

Clonality

Monoclonal

Clone number

145-2C11

Isotype

IgG

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd3e protein. The exact immunogen used to generate this antibody is proprietary information.

P22646

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-3 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed : 10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
See full target information Cd3d
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:641-651 PubMed40109863

2025

Astragalus polysaccharide enhances the therapeutic efficacy of cisplatin in triple-negative breast cancer through multiple mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

L I Sun,Shichao Zhuo,Xiaoxin Li,Husheng Kong,Weiwei DU,Chong Zhou,Junxing Huang

Heliyon 10:e27354 PubMed38509909

2024

C-type lectin 2D (CLEC2D) is upregulated in clear cell renal cell carcinoma (ccRCC) tissues and predicts poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huibing Li,Pengyi Zheng,Zhijun Li,Qingjiang Han,Bisheng Zhou,Kaixuan Wang
View all publications
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Product promise

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