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AB34722

FITC Anti-CD3 antibody [CD3-12]

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(7 Publications)

Rat Monoclonal CD3d antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse, Pig, Chicken, Monkey, Rhesus monkey, Human, Dog, Horse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CD3E.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CD3-12

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Horse, Chicken, Dog, Human, Pig, Monkey, Rhesus monkey

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human CD3E. The exact immunogen used to generate this antibody is proprietary information.

P07766

Specificity

ab34722 recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Chicken": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Horse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" }, "Rhesus monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/5 - 1/10", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Use 10ul of the suggested working dilution to label 10<sup>6</sup> cells in 100ul. Membrane permeabilisation is required for this application.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:109 PubMed40590998

2025

NGR-modified curcumin nanovesicles reverse immunotherapy resistance in triple-negative breast cancer via TLR9 and mTOR pathway modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Wang,Xiaoou Wang,Xinyu Zheng,Haiyang Jiang,Lu Liu,Ningye Ma,Xiaoshen Dong

Emerging microbes & infections 13:2396864 PubMed39331815

2024

A platform for the rapid screening of equine immunoglobins F (ab)2 derived from single equine memory B cells able to cross-neutralize to influenza virus.

Applications

Unspecified application

Species

Unspecified reactive species

Yuezhi Lin,Yayu Wang,Hongxin Li,Tong Liu,Jiaqi Zhang,Xing Guo,Wei Guo,Yaoxin Wang,Xiangning Liu,Shaoli Huang,Huaxin Liao,XiaoJun Wang

Biomedicines 12: PubMed39062070

2024

Newcastle Disease Virus Virotherapy: Unveiling Oncolytic Efficacy and Immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kawther A Zaher,Jehan S Alrahimi,Fatemah S Basingab,Alia M Aldahlawi

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Frontiers in immunology 15:1361531 PubMed38698849

2024

Infection of domestic pigs with a genotype II potent strain of ASFV causes cytokine storm and lymphocyte mass reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zuo,Guorui Peng,Junjie Zhao,Qizu Zhao,Yuanyuan Zhu,Yuan Xu,Lu Xu,Fangtao Li,Yingju Xia,Yebing Liu,Cheng Wang,Zhen Wang,Haidong Wang,Xingqi Zou

Science advances 9:eadh4057 PubMed37540755

2023

Single-dose mucosal replicon-particle vaccine protects against lethal Nipah virus infection up to 3 days after vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen R Welch,Jessica R Spengler,Sarah C Genzer,JoAnn D Coleman-McCray,Jessica R Harmon,Teresa E Sorvillo,Florine E M Scholte,Sergio E Rodriguez,T Justin O'Neal,Jana M Ritter,Georgia Ficarra,Katherine A Davies,Markus H Kainulainen,Elif Karaaslan,Éric Bergeron,Cynthia S Goldsmith,Michael K Lo,Stuart T Nichol,Joel M Montgomery,Christina F Spiropoulou

eLife 7: PubMed30102153

2018

CCR7 defines a precursor for murine iNKT cells in thymus and periphery.

Applications

Unspecified application

Species

Unspecified reactive species

Haiguang Wang,Kristin A Hogquist
View all publications

Product promise

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