AB34275

FITC Anti-CD3 antibody [UCHT1]

Name: FITC Anti-CD3 antibody [UCHT1] (ab34275) | Abcam
Brand: Abcam Limited
SKU: AB34275
Price: 85 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(15 Publications)

Mouse Monoclonal CD3d antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 15 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

3 Images

Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (AB34275)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (AB34275)

Flow Cytometry analysis of Human peripheral blood lymphocytes staining CD3 with ab34275 at 5μL (1.0μg) concentration (solid line), and isotype control Mouse IgG1 (FITC) at 1.0μg (dotted line).

Flow Cyt

AbReview29200****

Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (AB34275)

ab34275 staining CD3 in Human PBMCs by Flow Cytometry. PBMCs were isolated from buffy coat after Ficoll preparation in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy : Live Lymphocytes.

This image is courtesy of an anonymous Abreview

Flow Cyt

Lab

Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (AB34275)

FACS analysis of CD3 expression in human peripheral blood lymphocytes stained with ab34275 (solid line) at 0.25 ug and 0.25 ug isotype control (dashed line).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store undiluted

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:61 PubMed40399992

2025

Mechanism of LINC01018/miR-182-5p/Rab27B in the immune escape through PD-L1-mediated CD8 T cell suppression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hu,Guoshuo Chen,Aiping Luo,Hailin Zhao,Dan Li,Biao Peng,Jike Du,Dongdong Luo

BMC infectious diseases 24:229 PubMed38378486

2024

Chlamydia trachomatis enhances HPV persistence through immune modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Lu,Qi Wu,Li Wang,Lingting Ji

Journal of translational medicine 21:721 PubMed37838657

2023

Cancer stem cell-derived CHI3L1 activates the MAF/CTLA4 signaling pathway to promote immune escape in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Ji,Hao Yu,Dan Zhou,Xulong Fan,Yan Duan,Yijiang Tan,Min Lang,Guoli Shao

Experimental and therapeutic medicine 25:271 PubMed37206552

2023

Changes in the balance of Th17/Treg cells and oxidative stress markers in patients with HIV‑associated pulmonary tuberculosis who develop IRIS.

Applications

Unspecified application

Species

Unspecified reactive species

Long Wen,Lei Shi,Shan-Shan Wan,Tao Xu,Lei Zhang,Zhi-Guo Zhou

BMC immunology 23:58 PubMed36434505

2022

Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Can Cui,Dengwen Zhang,Ke Sun,Yi Zhu,Jindong Xu,Yin Kang,Guangyan Zhang,Yujin Cai,Songsong Mao,Ruichun Long,Jue Ma,Song Dong,Yi Sun

Cell transplantation 31:9636897221090257 PubMed35438589

2022

Cord Blood Natural Killer Cells Inhibit Sepsis Caused by Feces-Induced Acute Peritonitis via Increasing Endothelium Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Lan,Hong Zhang,Hui Zhao,Linghong Liu,Qing Shi,Dong Li,Xiuli Ju

Redox biology 50:102257 PubMed35149342

2022

T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Dang,Tianrun Li,Dongmin Yang,Guangxin Yang,Xing Du,Juan Yang,Yutong Miao,Lulu Han,Xiaolong Ma,Yuwei Song,Bo Liu,Xuan Li,Xian Wang,Juan Feng

Oncology letters 18:35-42 PubMed31289469

2019

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Niu Di,Yue Guo,Nannan Ding

Journal of controlled release : official journal of the Controlled Release Society 294:165-175 PubMed30557650

2018

Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice.

Applications

Unspecified application

Species

Unspecified reactive species

M Garofalo,A Villa,N Rizzi,L Kuryk,B Rinner,V Cerullo,M Yliperttula,V Mazzaferro,P Ciana

Viruses 10: PubMed30322158

2018

Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Mariangela Garofalo,Alessandro Villa,Nicoletta Rizzi,Lukasz Kuryk,Vincenzo Mazzaferro,Paolo Ciana

View all publications

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