AB24948

FITC Anti-CD3 epsilon antibody [C363.29B]

Name: FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948) | Abcam
Brand: Abcam Limited
SKU: AB24948
Price: 165 USD
Availability: InStock

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(5 Publications)

Rat Monoclonal CD3E antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

2 Images

Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (AB24948)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (AB24948)

Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 μg/10⁶ cells) and Rat Anti-Mouse CD19-PE.

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (AB24948)

ab24948 (3µg for 10⁶ cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.

578 PE

PE Anti-CD3 epsilon antibody [C363.29B]
667 PE/Cy5®

PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

C363.29B

Isotype

IgG2c

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Epitope

This antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg for 10^6 Cells", "FlowCyt-species-notes": "In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided). <a href='/en-us/products/primary-antibodies/fitc-rat-igg2c-kappa-rtk4174-isotype-control-ab114134'>ab114134</a> - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 17:2765-2770 PubMed35662227

2022

NR4A1 agonist cytosporone B attenuates neuroinflammation in a mouse model of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Zhen Yu,Bing-Qing Zhu,Lin Zhu,Shuo Li,Li-Mei Wang

Frontiers in immunology 13:828263 PubMed35251013

2022

Development of a Dendritic Cell/Tumor Cell Fusion Cell Membrane Nano-Vaccine for the Treatment of Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Wei Zhao,Jinke Huang,Fangxuan Li,Jindong Sheng,Hualin Song,Ying Chen

Nutrition & metabolism 16:49 PubMed31372176

2019

Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nurul Elyani Mohamad,Nadiah Abu,Swee Keong Yeap,Kian Lam Lim,Muhammad Firdaus Romli,Shaiful Adzni Sharifuddin,Kamariah Long,Noorjahan Banu Alitheen

BMC complementary and alternative medicine 17:344 PubMed28666436

2017

In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™.

Applications

Flow Cyt

Species

Mouse

Noraisyah Zulkawi,Kam Heng Ng,Rizi Zamberi,Swee Keong Yeap,Dilan Satharasinghe,Indu Bala Jaganath,Anisah Binti Jamaluddin,Sheau Wei Tan,Wan Yong Ho,Noorjahan Banu Alitheen,Kamariah Long

PLoS pathogens 6:e1001154 PubMed20976202

2010

Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Muraille,Pierre Gounon,Julie Cazareth,Johan Hoebeke,Christoph Lippuner,Ana Davalos-Misslitz,Toni Aebischer,Sylviane Muller,Nicolas Glaichenhaus,Evelyne Mougneau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD3 epsilon antibody [C363.29B]

Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (AB24948)

Flow Cytometry - FITC Anti-CD3 epsilon antibody [C363.29B] (AB24948)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise