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AB237453

FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

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(1 Publication)

Rabbit Recombinant Monoclonal CD3 zeta phospho Y83 antibody - conjugated to FITC. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain

2 Images
Flow Cytometry (Intracellular) - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237453)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237453)

Flow cytometry overlay histogram showing Jurkat + pervanadate ( 1mM, 30min)(Red) / Untreated control (Green) stained with ab237453. The cells were fixed with 4 % formaldehyde (10 min). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237453).

Isotype control antibody (Black) was Rabbit IgG, monoclonal [EPR25A] - Isotype Control (FITC) (ab223339) used at the same concentration and conditions as the primary antibody.

Unlabelled sample without incubation with primary antibody and secondary antibody (Blue) was also used as a control.

Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237453)
  • ICC

Lab

Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237453)

ab237453 staining CD3 zeta (phospho Y83) in Jurkat cells +/- pervanadate ( 1mM, 30min). The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, then incubated ab237453 at 1/50 dilution (shown in green). Counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • Unconjugated

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • Carrier free

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP776(2)Y

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB200317

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 1384049, PubMed : 1385158, PubMed : 2470098, PubMed : 7509083). CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed : 7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
See full target information CD247 phospho Y83
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2023:8215567 PubMed37035756

2023

T Cell Subsets and the Expression of Related MicroRNAs in Patients with Recurrent Early Pregnancy Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ni Yan,Jian Zhang,Na Yang,Chaochao Chen,Weiwei Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com