AB33858

FITC Anti-CD31 antibody [TLD-3A12]

Name: FITC Anti-CD31 antibody [TLD-3A12] (ab33858) | Abcam
Brand: Abcam Limited
SKU: AB33858
Price: 265 USD
Availability: InStock

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(29 Publications)

Mouse Monoclonal CD31 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Rat samples. Cited in 29 publications. Immunogen corresponding to Cell preparation containing Pecam1 protein.

View Alternative Names

CD31, Pecam, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

2 Images

Flow Cytometry - FITC Anti-CD31 antibody [TLD-3A12] (AB33858)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD31 antibody [TLD-3A12] (AB33858)

Figure A. ab33858 and RPE conjugated mouse IgG1 isotype control. Figure B. ab33858 and RPE conjugated mouse anti rat TCRa/b. All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD31 antibody [TLD-3A12] (AB33858)

Figure A. RPE conjugated mouse anti rat TCRa/b and FITC conjugated mouse IgG1 isotype control. Figure B. RPE conjugated mouse anti rat TCRa/b and ab33858. All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Unconjugated

Anti-CD31 antibody [TLD-3A12]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TLD-3A12

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Pecam1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q3SWT0

Specificity

Ab33858 recognises CD31 expressed primarily on endothelial cells, platelets and leucocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 10μl for labeling 106 cells in 100μl.<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 10μl for labeling 106 cells in 100μl.<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

This clone (TLD 3A12) has been shown to partially block the proliferative response of antigen specific CD4+ T cells to antigen presenting cells and relevant antigen.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Ab33858 was purified from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.

See full target information Pecam1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 16:e70058 PubMed40955870

2025

Preterm Birth Conditions Alter Muscle Stem Cells and Their Niche, Causing Lasting Impairments in Muscle Regeneration and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Alyson Deprez,Thomas Molina,Gael Cagnone,Pauline Garcia,Séverine Leclerc,Anik Cloutier,Rebecca Desaulniers,Benjamin Ellezam,Anne Monique Nuyt,Nicolas A Dumont

Journal of extracellular vesicles 13:e12434 PubMed38634538

2024

Functional diversity of apoptotic vesicle subpopulations from bone marrow mesenchymal stem cells in tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhao Zhang,Jian Yang,Shixing Ma,Xin Gao,Guanyu Wang,Yanping Sun,Yejia Yu,Zhuo Wang,Weidong Tian,Li Liao

Journal of ovarian research 17:52 PubMed38402185

2024

The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Biyao Jiang,Songshu Xiao,Shan Zhang,Fang Xiao

International journal of molecular sciences 25: PubMed38339124

2024

Utilization of the Rat Tibial Nerve Transection Model to Evaluate Cellular and Molecular Mechanisms Underpinning Denervation-Mediated Muscle Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Doherty,Monika Lodyga,Judy Correa,Caterina Di Ciano-Oliveira,Pamela J Plant,James R Bain,Jane Batt

Nature communications 14:7856 PubMed38030636

2023

A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjian Huang,Rong Yang,Jiao Jiao,Ze Li,Penghui Wang,Ye Liu,Sicheng Li,Canwen Chen,Zongan Li,Guiwen Qu,Kang Chen,Xiuwen Wu,Bo Chi,Jianan Ren

Acta pharmacologica Sinica 44:1841-1855 PubMed36973541

2023

Activating Wnt/β-catenin signaling by autophagic degradation of APC contributes to the osteoblast differentiation effect of soy isoflavone on osteoporotic mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ge,Ye-Jia Yu,Jia-Yi Li,Meng-Yu Li,Si-Mo Xia,Ke Xue,Shao-Yi Wang,Chi Yang

Redox biology 61:102649 PubMed36871183

2023

Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyu Li,Yejia Yu,Ke Xue,Jiayi Li,Geehun Son,Jiajia Wang,Wentao Qian,Shaoyi Wang,Jiawei Zheng,Chi Yang,Jing Ge

Frontiers in cell and developmental biology 11:971091 PubMed36910156

2023

Diversity of arterial cell and phenotypic heterogeneity induced by high-fat and high-cholesterol diet.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqi Wen,Rongsong Ling,Ruiyue Chen,Siyan Zhang,Yarong Dai,Tingtao Zhang,Fanyu Guo,Qingxin Wang,Guixin Wang,Yizhou Jiang

Progress in biomaterials 11:385-396 PubMed36271317

2022

Encapsulation of rat bone marrow-derived mesenchymal stem cells (rBMMSCs) in collagen type I containing platelet-rich plasma for osteoarthritis treatment in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shahidul Islam,Somayeh Ebrahimi-Barough,Mamun Al Mahtab,Sadegh Shirian,Hamid Reza Aghayan,Babak Arjmand,Amir Allahverdi,Faezeh Esmaeili Ranjbar,Amin Bigham Sadeg,Jafar Ai

International journal of stem cells 15:164-172 PubMed34711700

2021

Pharmaceutical Activation of Nrf2 Accelerates Diabetic Wound Healing by Exosomes from Bone Marrow Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Yuhui Cai,Qingrong Zhang,Yi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD31 antibody [TLD-3A12]

Flow Cytometry - FITC Anti-CD31 antibody [TLD-3A12] (AB33858)

Flow Cytometry - FITC Anti-CD31 antibody [TLD-3A12] (AB33858)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (29)

Product promise