FITC Anti-CD32B + CD32A antibody [AT10]

2 product images

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  Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

  Overlay histogram showing K562 cells stained with ab30356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30356, 1/5000 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (FITC Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype control ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

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  Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (ab30356)

  ab30356 staining CD32A + CD32B in K562 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab30356 at 1/50 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive signal under the same testing conditions in K562 cells fixed with 80% methanol (5 min).