AB30356

FITC Anti-CD32B + CD32A antibody [AT10]

Name: Anti-CD32B + CD32A FITC antibody [AT10] (ab30356) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB30356
Price: 250 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal FCG2B antibody - conjugated to FITC. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

2 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ab30356 staining CD32A + CD32B in K562 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab30356 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in K562 cells fixed with 80% methanol (5 min).

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Overlay histogram showing K562 cells stained with ab30356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30356, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

578 PE

PE Anti-CD32B + CD32A antibody [AT10]
Biotin

Biotin Anti-CD32B + CD32A antibody [AT10]
Unconjugated

Anti-CD32B + CD32A antibody [AT10] - BSA free
Unconjugated

Anti-CD32B + CD32A antibody [AT10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT10

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B

Additional targets

FCGR2A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 22:87 PubMed26475492

2015

FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Shiang-Jong Tzeng,Wan-Yu Li,Hui-Ying Wang

Veterinary immunology and immunopathology 61:181-93 PubMed9613433

1998

Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

I Lilliehöök,A Johannisson,L Håkansson

Blood 91:2369-80 PubMed9516136

1998

Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene.

Applications

Unspecified application

Species

Unspecified reactive species

D Metes,L K Ernst,W H Chambers,A Sulica,R B Herberman,P A Morel

Molecular immunology 28:1243-54 PubMed1835758

1991

Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

J Greenman,A L Tutt,A J George,K A Pulford,G T Stevenson,M J Glennie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD32B + CD32A antibody [AT10]

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (4)

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