JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB59474

FITC Anti-CD4 antibody [B-A1]

3

(4 Reviews)

|

(12 Publications)

Mouse Monoclonal CD4 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing CD4 protein.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

2 Images
Flow Cytometry - FITC Anti-CD4 antibody [B-A1] (AB59474)
  • Flow Cyt

AbReview33821****

Flow Cytometry - FITC Anti-CD4 antibody [B-A1] (AB59474)

Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

Gating Strategy : Live lymphocytes

This image was generated using the ascites version of the product.

This image is courtesy of an anonymous Abreview

Flow Cytometry - FITC Anti-CD4 antibody [B-A1] (AB59474)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD4 antibody [B-A1] (AB59474)

A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-A1

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD4 protein. The exact immunogen used to generate this antibody is proprietary information.

P01730

Specificity

Ab59474 recognises a 55 kDa protein.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by affinity chromatography.
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS, 5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
style
left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:278 PubMed40819077

2025

Targeting LINC02544/miR-497-5p/CAPRIN1 axis via exosome-based siRNA to overcome immunotherapy resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lian,Jiayi Li,Shihui Tang,Ting Li,Jinping Li

Aging and disease : PubMed40249929

2025

Plasma Interferon-gamma is Associated with Poor Treatment Response in Neovascular Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Kai Thomsen,Maria Abildgaard Steffensen,Jenni Martinez Villarruel Hinnerskov,Amalie Thomsen Nielsen,Henrik Vorum,Bent Honoré,Mogens Holst Nissen,Torben Lykke Sørensen

Translational oncology 53:102310 PubMed39929064

2025

CSF3 promotes colorectal cancer progression by activating p65/NF-κB signaling pathway and inducing an immunosuppressive microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Xu,Na Li,Hui Xie,Changwei Duan,Xingchen Liao,Ruoran Li,Heng Zhang,Yuanming Pan,Xianzong Ma,Shuwen Du,Jianqiu Sheng,Xin Wang,Lang Yang,Peng Jin

Immunity, inflammation and disease 10:e727 PubMed36301031

2022

Forkhead box M1 recruits FoxP3 Treg cells to induce immune escape in hilar cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ma,Zhaowei Sun,Xueliang Li,Jingyun Guo,Qinlei Wang,Mujian Teng

International archives of allergy and immunology 184:85-97 PubMed36265457

2022

Changes of T Lymphocyte Subsets in Peripheral Blood of Patients with Intermediate and Advanced Cervical Cancer before and after Nimotuzumab Combined with Chemoradiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Teng,Guimin Cui,Ligang Qian,Lujun Zhao

International journal of molecular medicine 48: PubMed34036377

2021

Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR‑145‑3p/NFATc2/NF‑κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Can Cui,Dengwen Zhang,Ke Sun,Haifeng Li,Liqian Xu,Gen Lin,Yuanbo Guo,Jiaqi Hu,Jieyuan Chen,Lidan Nong,Yujin Cai,Dongnan Yu,Wei Yang,Peng Wang,Yi Sun

Frontiers in oncology 11:595609 PubMed33777742

2021

Circular RNA CHST15 Sponges miR-155-5p and miR-194-5p to Promote the Immune Escape of Lung Cancer Cells Mediated by PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianru Yang,Yang Jia,Bing Wang,Shengrong Yang,Kun Du,Yujie Luo,Yunhe Li,Bing Zhu

Frontiers in immunology 11:1934 PubMed33013847

2020

Mechanisms of M2 Macrophage-Derived Exosomal Long Non-coding RNA PVT1 in Regulating Th17 Cell Response in Experimental Autoimmune Encephalomyelitisa.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Jinjin Xia,Donghui Li,Ying Kang,Wei Fang,Peng Huang

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

Oncology letters 18:35-42 PubMed31289469

2019

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Niu Di,Yue Guo,Nannan Ding
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

  • 4
  • 2
Primary Antibodies

AB181724

Anti-CD4 antibody [EPR6855] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] - BSA and Azide free (AB181724)

  • 4
  • 9
Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Flow Cytometry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

  • 0
  • 0
Primary Antibodies

AB221775

Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19
Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

  • 0
  • 0

Complementary Products

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB307335

Alexa Fluor® 555 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3 antibody [SP162] (AB307335)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB199397

PE Anti-ZNFN1A2/HELIOS antibody [22F6]

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com