AB18281

FITC Anti-CD4 antibody [MEM-241]

Name: Anti-CD4 FITC antibody [MEM-241] (ab18281) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB18281
Price: 95 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal CD4 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD4.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images

Flow Cytometry - FITC Anti-CD4 antibody [MEM-241] (AB18281)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD4 antibody [MEM-241] (AB18281)

Overlay histogram showing peripheral blood lymphocytes stained with ab18281 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18281, 0.5 μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 μg/1x10⁶ cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-241

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD4. The exact immunogen used to generate this antibody is proprietary information.

P01730

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4.15 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC immunology 23:58 PubMed36434505

2022

Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Can Cui,Dengwen Zhang,Ke Sun,Yi Zhu,Jindong Xu,Yin Kang,Guangyan Zhang,Yujin Cai,Songsong Mao,Ruichun Long,Jue Ma,Song Dong,Yi Sun

Cancer cell international 21:58 PubMed33468159

2021

IRF4 overexpression promotes the transdifferentiation of tregs into macrophage-like cells to inhibit the development of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Wang,Song Li,Honglang Li,Xiaoshuang Zhou,Huabin Wen,Bin Lai

eLife 9: PubMed32441251

2020

Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Franziska Eckert,Peng Gao,Janine Wesslowski,Xianxian Wang,Jasmijn Rath,Karin Nienhaus,Gary Davidson,Gerd Ulrich Nienhaus

View all publications

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