AB239230

FITC Anti-CD4 antibody [OX-35]

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal CD4 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD4 protein.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

2 Images

Flow Cytometry - FITC Anti-CD4 antibody [OX-35] (AB239230)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD4 antibody [OX-35] (AB239230)

Flow cytometric analysis of rat thymocytes labeling CD4 with ab239230. Surface staining. Gated on thymocytes.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD4 antibody [OX-35] (AB239230)

Flow cytometry analysis of rat thymocytes stained using ab239230 (concentration in sample 1 µg/ml) showing separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-35

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD4 protein. The exact immunogen used to generate this antibody is proprietary information.

P01730

Epitope

Extracellular.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40571642

2025

Modulation of Th17/Treg Balance by Total Flavonoids from Semen Cuscutae through PGRMC2 in a Diminished Ovarian Reserve Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhou,Shanshan Xu,Juan Tang,Xiaomei Han,Yu Jiang,Huaying Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402368 PubMed39031576

2024

A Glucose-Responsive Hydrogel Inhibits Primary and Secondary BRB Injury for Retinal Microenvironment Remodeling in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhou,Chan Zhao,Zhiyuan Shi,Zbynek Heger,HuaQing Jing,Zhengming Shi,Yunsheng Dou,Siyu Wang,Zitong Qiu,Nan Li

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

Primary Antibodies

AB181724

Anti-CD4 antibody [EPR6855] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] - BSA and Azide free (AB181724)

Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Flow Cytometry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

Primary Antibodies

AB221775

Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

Complementary Products

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

Primary Antibodies

AB307335

Alexa Fluor® 555 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3 antibody [SP162] (AB307335)

Primary Antibodies

AB242075

violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5]

Flow Cytometry - violetFluor™ 450 Anti-IL-2 Receptor alpha antibody [PC61.5] (AB242075)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com