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AB210349

FITC Anti-CD4 antibody [RM4-5]

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(3 Publications)

Rat Monoclonal CD4 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Flow Cytometry - FITC Anti-CD4 antibody [RM4-5] (AB210349)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD4 antibody [RM4-5] (AB210349)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.25 μg ab210349 (solid line), compared with 0.25 μg FITC Rat IgG2a (dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM4-5

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.25 μg.</p>" } } }
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Product details

The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will "compete" for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab210349 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed39683500

2024

Omega-3 Fatty Acids Weaken Lymphocyte Inflammatory Features and Improve Glycemic Control in Nonobese Diabetic Goto-Kakizaki Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tiago Bertola Lobato,Elvirah Samantha de Sousa Santos,Patrícia Nancy Iser-Bem,Henrique de Souza Falcão,Gabriela Mandú Gimenes,Janaina Ribeiro Barbosa Pauferro,Glayce Tavares Rodrigues,Ilana Souza Correa,Ana Carolina Gomes Pereira,Maria Elizabeth Pereira Passos,João Carlos de Oliveira Borges,Amara Cassandra Dos Anjos Alves,Camila Soares Dos Santos,Maria Janaina Leite de Araújo,Vinícius Leonardo Sousa Diniz,Adriana Cristina Levada-Pires,Tânia Cristina Pithon-Curi,Laureane Nunes Masi,Rui Curi,Sandro Massao Hirabara,Renata Gorjão

FEBS letters 598:2566-2580 PubMed39095330

2024

Insulin resistance in nonobese type 2 diabetic Goto Kakizaki rats is associated with a proinflammatory T lymphocyte profile.

Applications

Unspecified application

Species

Unspecified reactive species

Tiago Bertola Lobato,Richelieau Manoel,Ana Carolina Gomes Pereira,Ilana Souza Correa,Patrícia Nancy Iser-Bem,Elvirah Samantha de Sousa Santos,Joice Naiara Bertaglia Pereira,Maria Janaína Leite de Araújo,João Carlos de Oliveira Borges,Janaina Ribeiro Barbosa Pauferro,Vinicius Leonardo Sousa Diniz,Maria Vitória Martins Scervino,Tamires Duarte Serdan,Tania Cristina Pithon-Curi,Laureane Nunes Masi,Sandro Massao Hirabara,Rui Curi,Renata Gorjão

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong
View all publications
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