Skip to main content

Rabbit Recombinant Monoclonal CD40 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - FITC Anti-CD40 antibody [EPR20735] (AB314949), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/2500

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Version

Target data

Function

Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD40 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20735

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - FITC Anti-CD40 antibody [EPR20735] (ab314949), expandable thumbnail

    Flow Cytometry - FITC Anti-CD40 antibody [EPR20735] (ab314949)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314949 (right) or Rabbit IgG [EPR25A] FITC (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314949 or Rabbit IgG [EPR25A] FITC (1x 106in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD19.

    Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable lymphocytes.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com