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AB25064

FITC Anti-CD44 antibody [KM201]

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(11 Publications)

Rat Monoclonal CD44 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 11 publications.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

1 Images
Flow Cytometry - FITC Anti-CD44 antibody [KM201] (AB25064)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD44 antibody [KM201] (AB25064)

ab25064 (at a concentration of 1 μg/106 cells) staining cells from BALB/c bone were stained by flow cytometry. Large cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KM201

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Epitope

Reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.

Specificity

Recognises all isoforms of CD44/pgp-1 (Mr 80-95 kDa)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg1-kappa-rtk2071-isotype-control-ab18404'>ab18404</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

ab25064 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD44
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou

Immunotherapy 16:975-985 PubMed39115961

2024

The development of anti-PD-1 antibody-induced spinal cord injury in bone marrow transplant C57BL/6 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Huachun Chen,Zhouxiao Lu,Xiaowei Ni,Hui Zhang,Guiyuan Chen,Xiaoyu Wu,Mingxing Ding

Research in pharmaceutical sciences 17:123-133 PubMed35280837

2022

Comparison of the efficacy of bone morphogenetic protein-4 on differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Hosseinzadeh Shirzeyli,Ali Tayyebiazar,Fereshteh Aliakbari,Fahimeh Ghasemi,Fatemeh Eini,Farhad Hosseinzadeh Shirzeyli,Elmira Vanaki,Aligholi Sobhani

Cell death discovery 8:2 PubMed35013126

2022

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhang Fan,Shiming Li,Dawei Wang

Molecular medicine reports 22:2767-2774 PubMed32945380

2020

Role of the HIF‑1α/SDF‑1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghua Xue,Zhikun Li,Yi Wang,Xiaodong Zhu,Ruixi Hu,Wei Xu

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

Journal of cellular physiology 234:23485-23494 PubMed31206187

2019

Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guangjie Li,Jiangdong An,Xingwen Han,Xueliang Zhang,Wenjin Wang,Shuanke Wang

Microscopy research and technique 80:1151-1160 PubMed28921810

2017

Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam H Shirzeyli,Neda Khanlarkhani,Fardin Amidi,Farshad H Shirzeyli,Fereydoon S Aval,Aligholi Sobhani

Iranian journal of reproductive medicine 11:965-76 PubMed24639722

2014

Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells.

Applications

Flow Cyt

Species

Mouse

Maryam Hosseinzadeh Shirzeily,Parichehr Pasbakhsh,Fardin Amidi,Kobra Mehrannia,Aligholi Sobhani

Biomaterials 33:7849-57 PubMed22835643

2012

Synergistic effects of SDF-1α chemokine and hyaluronic acid release from degradable hydrogels on directing bone marrow derived cell homing to the myocardium.

Applications

BL

Species

Unspecified reactive species

Brendan P Purcell,Jeremy A Elser,Anbin Mu,Kenneth B Margulies,Jason A Burdick
View all publications
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