AB27287

FITC Anti-CD45 antibody [B-A11]

Name: FITC Anti-CD45 antibody [B-A11] (ab27287) | Abcam
Brand: Abcam Limited
SKU: AB27287
Price: 155 USD
Availability: InStock

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(34 Publications)

Mouse Monoclonal CD45 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 34 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (AB27287)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (AB27287)

A typical lymphocytes staining pattern with ab27287.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-A11

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

Ab27287 recognises the Leucocyte Common Antigen (LCA) with different isoforms of 180-190-205-220 kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use 10 μl to label 106 cells or 100 μl of whole blood.<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS, 5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and biophysics 83:1771-1783 PubMed39681812

2024

METTL3 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells Under the Inflammatory Microenvironment Through the miR-141-3p/ZEB1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weijia Li,Adili Alimujiang

Journal of orthopaedic surgery and research 18:876 PubMed37980502

2023

BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ning,Huihui Guo,Mingming Ma,Zhengang Zha

Journal of periodontal research 58:1235-1247 PubMed37712743

2023

Long non-coding RNA X-Inactive Specific Transcript (XIST) interacting with USF2 promotes osteogenic differentiation of periodontal ligament stem cells through regulation of WDR72 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Ya-Dong Li,Liu-Yang Ren,Hai-Long Song,Qiao-Yun Yang,Dong-Liang Xu

Investigative ophthalmology & visual science 64:21 PubMed36715672

2023

Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Emma N Finburgh,Olivier Mauduit,Takako Noguchi,Jennifer J Bu,Anser A Abbas,Dominic F Hakim,Saverio Bellusci,Robyn Meech,Helen P Makarenkova,Natalie A Afshari

Stem cells international 2022:2164057 PubMed36311041

2022

miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baodong Wang,Na Xu,Li Cao,Xiaojun Yu,Shanxi Wang,Qikun Liu,Yinguang Wang,Haoran Xu,Yang Cao

NPJ Regenerative medicine 7:53 PubMed36114225

2022

Chondrogenic primed extracellular vesicles activate miR-455/SOX11/FOXO axis for cartilage regeneration and osteoarthritis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Sun,Jie Zhao,Qiang Wu,Yuxin Zhang,Yongqing You,Wenbo Jiang,Kerong Dai

Tissue engineering and regenerative medicine 19:1321-1336 PubMed36074328

2022

The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Yong Huang,Daqian Liu,Teng Zeng,Jingzhe Wang,Ang Li,Dawei Wang,Xiaoyu Wang

Oxidative medicine and cellular longevity 2022:5772509 PubMed36105481

2022

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Youde Liang,Ruiping Zhou,Xin Liu,Lin You,Chang Chen,Xiaoling Ye,Wei Wei,Jie Liu,Jiawei Dai,Kaixiong Li,Xiangxiang Zhao

Cell journal 24:449-457 PubMed36093804

2022

Differentiation of Alginate-Encapsulated Wharton Jelly-Derived Mesenchymal Stem Cells into Insulin Producing Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Poursafavi,Saeid Abroun,Saeid Kaviani,Nasim Hayati Roodbari

Cell death & disease 13:535 PubMed35672285

2022

Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Jun Wang,Zhen-Zhen Qiu,Fu-Wei Chen,An-Li Mao,Jun-Chao Bai,Ye-Jing Hong,Zhong-Pan Zhang,Wu-An Zhu,Zhi-Wei Zhang,Hao Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD45 antibody [B-A11]

Flow Cytometry - FITC Anti-CD45 antibody [B-A11] (AB27287)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

Product promise