AB24897

FITC Anti-CD45R antibody [RA3-6B2]

Name: Anti-CD45R FITC antibody [RA3-6B2] (ab24897) Rat monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB24897
Price: 525 USD
Availability: InStock

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(2 Publications)

Rat Monoclonal CD45 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry - FITC Anti-CD45R antibody [RA3-6B2] (AB24897)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD45R antibody [RA3-6B2] (AB24897)

Flow cytometry of C57Bl/6 splenocytes labeling CD45R with ab24897 at 0.25 μg (solid line) or Rat IgG2a FITC isotype control at 0.25 μg (dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RA3-6B2

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

In vivo administration of ab24897 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45R also known as Leukocyte Common Antigen or LCA is a protein that plays a significant role in immune cell function. It is an isoform of the larger protein CD45 which includes several alternatively spliced variants. CD45R is a transmembrane protein tyrosine phosphatase with a mass ranging from 180 to 220 kDa depending on its isoform. This protein is primarily expressed on hematopoietic cells including T cells B cells and other leukocytes. It functions mechanically by modulating signaling thresholds essential for lymphocyte development and activation.

Biological function summary

CD45R influences signaling pathways critical for immune response regulation. It does not form a complex by itself but interacts with other membrane receptors and cytoplasmic kinases to mediate signal transduction. The protein adjusts the activity of Src family kinases through its phosphatase activity impacting cell-cell communication and responsive behavior to antigens. CD45R is involved in maintaining a functional immune surveillance system particularly ensuring the adequate responsiveness of lymphocytes.

Pathways

CD45R's phosphatase activity is integral to the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. This activity regulates Lck and Fyn kinases within these pathways contributing to proper lymphocyte activation and development. Any disruption in CD45R function can affect these pathways potentially impacting proteins like ZAP-70 and Syk which are essential for signal transduction downstream of the TCR and BCR respectively.

CD45R has been implicated in conditions such as severe combined immunodeficiency (SCID) and autoimmune diseases like multiple sclerosis. Its malfunction or aberrant expression can cause improper immune system activation or suppression. In autoimmune contexts CD45R's interaction with other proteins like CTLA-4 may become dysregulated leading to immune system attacks on the body's tissues. Understanding these connections helps in developing therapeutic strategies targeting immune dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 33:108236 PubMed33027652

2020

Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Vahid Hoghooghi,Alexandra L Palmer,Ariana Frederick,Yulan Jiang,Jessica E Merkens,Anjali Balakrishnan,Trisha M Finlay,Anders Grubb,Efrat Levy,Paul Gordon,Frank R Jirik,Minh Dang Nguyen,Carol Schuurmans,Frank Visser,Shannon E Dunn,Shalina S Ousman

European journal of microbiology & immunology 4:123-7 PubMed24883198

2014

Evaluation of immune responses by live infectious bursal disease vaccines to avoid vaccination failures.

Applications

Unspecified application

Species

Unspecified reactive species

P Jakka,Y K Reddy,J J Kirubaharan,N D J Chandran

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-CD45R antibody [RA3-6B2]

Flow Cytometry - FITC Anti-CD45R antibody [RA3-6B2] (AB24897)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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