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AB326487

FITC Anti-CD45R0 antibody [UCHL1]

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Mouse Monoclonal CD45 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

3 Images
Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326487 antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)

Separation of human CD45R0 positive CD45RA negative lymphocytes (red-filled) from CD45R0 negative CDCD45RA positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326487 (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD45R0 antibody [UCHL1] (AB326487)

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD45RA (MEM-56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and ab326487 (20 μl reagent / 100 μl of peripheral whole blood).

  • Unconjugated

    Anti-CD45RO antibody [UCHL1]

  • 660 APC

    APC Anti-CD45R0 antibody [UCHL1]

  • 519 FITC

    FITC Anti-CD45RO antibody [UCHL1]

  • 578 PE

    PE Anti-CD45R0 antibody [UCHL1]

  • 578 PE

    PE Anti-CD45RO antibody [UCHL1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD45RO antibody [UCHL1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD45RO antibody [UCHL1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD45RO antibody [UCHL1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD45RO antibody [UCHL1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHL1

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody UCHL1 recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Product promise

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