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Mouse Monoclonal CD48 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD48 protein.

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Images

Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885), expandable thumbnail
  • Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: BSA, PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CD48 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P09326

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 20μl per 100μl whole blood.

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

2 products for Alternative Product

Target data

Function

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817).

Alternative names

CD48, BCM1, BLAST1, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, SLAMF2

Recommended products

Mouse Monoclonal CD48 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD48 protein.

Key facts

Isotype
IgG1
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CD48 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P09326
Clone number
MEM-102
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD48 also known as SLAMF2 or B-lymphocyte activation marker BLAST-1 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass approximately 45-70 kDa depending on glycosylation. It is expressed on the surface of various immune cells like T cells B cells and natural killer (NK) cells. CD48 does not possess an intracellular signaling domain but engages in extracellular interactions with other proteins affecting immune cell activities.

Biological function summary

CD48 serves as a co-stimulatory molecule involved in immune response modulation. It interacts with 2B4 (CD244) a member of the signaling lymphocyte activation molecule (SLAM) family to form a receptor-ligand complex. This interaction can result in enhanced T cell and NK cell responses. CD48's presence on activated hematopoietic and non-hematopoietic cells highlights its role in cell-to-cell communication.

Pathways

CD48 participates in SLAM family receptor signaling and natural killer cell-mediated cytotoxicity. In the SLAM pathway CD48 enhances communication and regulation between T cells and NK cells. This communication also relates to the roles of CD244 linking it to downstream signaling pathways that influence immune cell function. These pathways illustrate the critical network CD48 is part of which impacts various immune responses.

Associated diseases and disorders

CD48 is involved in autoimmune conditions like rheumatoid arthritis and immunological cancers such as lymphoma. In rheumatoid arthritis CD48 along with CD244 modulates immune responses which can exacerbate inflammation. In lymphoma abnormal CD48 expression can contribute to tumor progression and immune evasion indicating its potential as a therapeutic target.

Product promise

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2 product images

  • Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (ab21885), expandable thumbnail

    Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (ab21885)

    Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD48 antibody [MEM-102] (ab21885) at 20 µL/100 µL of peripheral whole blood.

  • Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (ab21885), expandable thumbnail

    Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (ab21885)

    Flow cytometry analysis (surface staining) of human peripheral whole blood stained using Anti-CD48 antibody [MEM-102] (ab21885) (20 µL reagent/100 µL of peripheral whole blood). CD48 positive lymphocytes (red-filled). CD48 negative neutrophil granulocytes (black-dashed).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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