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AB21885

FITC Anti-CD48 antibody [MEM-102]

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(1 Publication)

Mouse Monoclonal CD48 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD48 protein.

View Alternative Names

CD48, BCM1, BLAST1, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, SLAMF2

2 Images
Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885)

Flow cytometry analysis (surface staining) of human peripheral whole blood stained using Anti-CD48 antibody [MEM-102] (ab21885) (20 µL reagent/100 µL of peripheral whole blood). CD48 positive lymphocytes (red-filled). CD48 negative neutrophil granulocytes (black-dashed).

Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD48 antibody [MEM-102] (AB21885)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD48 antibody [MEM-102] (ab21885) at 20 µL/100 µL of peripheral whole blood.

  • Unconjugated

    Anti-CD48 antibody [MEM-102]

  • Biotin

    Biotin Anti-CD48 antibody [MEM-102]

  • 660 APC

    APC Anti-CD48 antibody [MEM-102]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-102

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD48 protein. The exact immunogen used to generate this antibody is proprietary information.

P09326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 20μl per 100μl whole blood.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD48 also known as SLAMF2 or B-lymphocyte activation marker BLAST-1 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass approximately 45-70 kDa depending on glycosylation. It is expressed on the surface of various immune cells like T cells B cells and natural killer (NK) cells. CD48 does not possess an intracellular signaling domain but engages in extracellular interactions with other proteins affecting immune cell activities.
Biological function summary

CD48 serves as a co-stimulatory molecule involved in immune response modulation. It interacts with 2B4 (CD244) a member of the signaling lymphocyte activation molecule (SLAM) family to form a receptor-ligand complex. This interaction can result in enhanced T cell and NK cell responses. CD48's presence on activated hematopoietic and non-hematopoietic cells highlights its role in cell-to-cell communication.

Pathways

CD48 participates in SLAM family receptor signaling and natural killer cell-mediated cytotoxicity. In the SLAM pathway CD48 enhances communication and regulation between T cells and NK cells. This communication also relates to the roles of CD244 linking it to downstream signaling pathways that influence immune cell function. These pathways illustrate the critical network CD48 is part of which impacts various immune responses.

CD48 is involved in autoimmune conditions like rheumatoid arthritis and immunological cancers such as lymphoma. In rheumatoid arthritis CD48 along with CD244 modulates immune responses which can exacerbate inflammation. In lymphoma abnormal CD48 expression can contribute to tumor progression and immune evasion indicating its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed : 12007789, PubMed : 19494291, PubMed : 27249817, PubMed : 9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed : 19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed : 12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed : 27249817).
See full target information CD48
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Plant physiology and biochemistry : PPB 140:78-87 PubMed31085449

2019

Heat stress reveals high molecular mass proteasomes in Arabidopsis thaliana suspension cells cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Aristizábal,Viridiana Rivas,Gladys I Cassab,Fernando Lledías
View all publications
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