Mouse Monoclonal CD53 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD53 protein.
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![Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (AB68565), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd53-antibody-mem-53-ab68565--flow-cytometry-img55190.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (AB68565)")
Publications
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- Journal of immunology (Baltimore, Md. : 1950) 153:4997-50071994Cross-linking of CD53 promotes activation of resting human B lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA M Rasmussen,H K Blomhoff,T Stokke,V Horejsi,E B SmelandPubMed 7963560
- Folia biologica 35:289-971989Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.Applications:Unspecified applicationReactive species:Unspecified reactive speciesV Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,A Bukovský,V HorejsíPubMed 2534480
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD53 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P19397
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes Use neat ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:32974937, PubMed:35767951). Plays a role in the activation of monocytes and B-cells (PubMed:8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed:28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity).
Alternative names
CD53, MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25
Recommended products
Mouse Monoclonal CD53 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD53 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD53 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P19397
- Clone number
- MEM-53
- Purification technique
- Size-exclusion chromatography
- Specificity
ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD53 also known as Tetraspanin-25 is a transmembrane glycoprotein with a molecular mass of approximately 35-40 kDa. It belongs to the tetraspanin family characterized by four transmembrane domains. CD53 primarily resides on the surface of leukocytes including B cells T cells natural killer cells and macrophages. This protein plays an important role in signal transduction processes by organizing proteins on the cell membrane and forming tetraspanin-enriched microdomains.
- Biological function summary
CD53 plays a significant role in regulating immune responses cell development and migration. It does not work alone but as part of larger protein complexes that influence cell communication and inter-organizational responses. CD53 participates in organizing membrane proteins into functional complexes which impacts cellular processes such as adhesion signaling and cellular activation. Its interaction with other proteins is critical to maintain normal immune system functionality and responding appropriately to infections.
- Pathways
CD53 is involved in the regulation of immune signaling and cellular communication networks. It is a component of the tetraspanin web which influences the integrin and immunoreceptor pathways two important signaling pathways in cellular communication. Proteins such as CD9 and CD81 also members of the tetraspanin family frequently interact with CD53 suggesting cooperative roles in modulating cellular adhesion and trafficking processes critical for immune response.
- Associated diseases and disorders
CD53 shows a connection to immune system dysfunctions including autoimmune conditions and certain types of cancer. Its altered expression can contribute to abnormal immune responses seen in diseases like systemic lupus erythematosus (SLE) and lymphoma. In these disorders CD53's interaction with CD19 and other B-cell-associated proteins plays a part in abnormal cell signaling leading to issues in cell proliferation or miscommunication within the immune system.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565), expandable thumbnail](https://content.abcam.com/products/images/fitc-cd53-antibody-mem-53-ab68565--flow-cytometry-img55190.jpg "Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565)")
Flow Cytometry - FITC Anti-CD53 antibody [MEM-53] (ab68565)
Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
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