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AB18237

FITC Anti-CD59 antibody [MEM-43]

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(9 Publications)

Mouse Monoclonal CD59 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing CD59 protein.

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

1 Images
Flow Cytometry - FITC Anti-CD59 antibody [MEM-43] (AB18237)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD59 antibody [MEM-43] (AB18237)

Flow cytometry analysis staining CD59 in HL-60 cells using ab18237 at a 1μg/ml dilution. Sp2 cells were used as the negative control.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-43

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information.

P13987

Specificity

The antibody reacts with a well defined epitope (W40, R-53) on CD59 molecule.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>20 μl for a 100 μl sample is recommended.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.
See full target information CD59
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Carbohydrate polymers 180:376-384 PubMed29103517

2017

Stability and bioactivity of chitosan as a transfection agent in primary human cell cultures: A case for chitosan-only controls.

Applications

Unspecified application

Species

Unspecified reactive species

Tanya L Cupino,Billy A Watson,Alan C Cupino,Keiji Oda,Mark G Ghamsary,Salvador Soriano,Wolff M Kirsch

Journal of virology 87:11148-59 PubMed23926339

2013

6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaisa-Emilia Makkonen,Paula Turkki,Johanna P Laakkonen,Seppo Ylä-Herttuala,Varpu Marjomäki,Kari J Airenne

The Journal of biological chemistry 286:20952-62 PubMed21507937

2011

Mapping the intermedilysin-human CD59 receptor interface reveals a deep correspondence with the binding site on CD59 for complement binding proteins C8alpha and C9.

Applications

FuncS

Species

Human

Stephanie E Wickham,Eileen M Hotze,Allison J Farrand,Galina Polekhina,Tracy L Nero,Stephen Tomlinson,Michael W Parker,Rodney K Tweten

The Journal of biological chemistry 284:12719-26 PubMed19293153

2009

Intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis.

Applications

Unspecified application

Species

Human

Stephanie LaChapelle,Rodney K Tweten,Eileen M Hotze

Biochemical and biophysical research communications 243:706-10 PubMed9500981

1998

Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

M Cebecauer,J Cerný,V Horejsí

Journal of immunology (Baltimore, Md. : 1950) 149:2262-70 PubMed1382093

1992

The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.

Applications

Unspecified application

Species

Unspecified reactive species

T Cinek,V Horejsí

Science (New York, N.Y.) 254:1016-9 PubMed1719635

1991

GPI-anchored cell-surface molecules complexed to protein tyrosine kinases.

Applications

Unspecified application

Species

Unspecified reactive species

I Stefanová,V Horejsí,I J Ansotegui,W Knapp,H Stockinger

Molecular immunology 26:153-61 PubMed2918859

1989

Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.

Applications

Unspecified application

Species

Unspecified reactive species

I Stefanová,I Hilgert,H Kristofová,R Brown,M G Low,V Horejsí

Immunogenetics 30:188-93 PubMed2476389

1989

Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11.

Applications

Unspecified application

Species

Unspecified reactive species

U H Forsberg,V Bazil,I Stefanová,J Schröder
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