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AB95140

FITC Anti-CD62L antibody [DREG-56]

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(7 Publications)

Mouse Monoclonal CD62L antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing SELL protein.

View Alternative Names

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1

2 Images
Flow Cytometry - FITC Anti-CD62L antibody [DREG-56] (AB95140)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD62L antibody [DREG-56] (AB95140)

Flow cytometry surface staining of CD62L in human peripheral blood using ab95140 at (20 µL reagent/100 µL of peripheral whole blood).

Flow Cytometry - FITC Anti-CD62L antibody [DREG-56] (AB95140)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD62L antibody [DREG-56] (AB95140)

Flow Cytometry analysis (surface staining) of CD62L in human peripheral whole blood stained using ab95140 at (20 µL reagent/100 µL of peripheral whole blood). CD62L positive human neutrophil granulocytes (red-filled) and CD62L negative lymphocytes (black-dashed).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DREG-56

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing SELL protein. The exact immunogen used to generate this antibody is proprietary information.

P14151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>20 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension</p>" } } }
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Product details

Free of unconjugated FITC.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).
See full target information SELL
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:6268-6276 PubMed36247260

2022

Th17 cells regulate the progress of anti-NMDAR encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chaosheng Zeng,Li Li,Lin Chen,Pengxiang Li,Min Chen,Xiaowen Wu,Cong Chen

The Journal of biological chemistry 284:8833-45 PubMed19129194

2009

In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.

Applications

Unspecified application

Species

Unspecified reactive species

David J Killock,Maddy Parsons,Marouan Zarrouk,Simon M Ameer-Beg,Anne J Ridley,Dorian O Haskard,Marketa Zvelebil,Aleksandar Ivetic

Journal of leukocyte biology 84:1192-201 PubMed18653462

2008

Critical role of Lck in L-selectin signaling induced by sulfatides engagement.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xu,Liang Chen,Xin Shang,Lingling Cui,Jixian Luo,Cuixia Chen,Xueqing Ba,Xianlu Zeng

Journal of immunology (Baltimore, Md. : 1950) 169:1768-73 PubMed12165498

2002

L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Mark A Jutila,Sandy Kurk,Larrisa Jackiw,Randall N Knibbs,Lloyd M Stoolman

American journal of respiratory and critical care medicine 159:1205-14 PubMed10194167

1999

Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep.

Applications

Unspecified application

Species

Unspecified reactive species

W M Abraham,A Ahmed,J R Sabater,I T Lauredo,Y Botvinnikova,R J Bjercke,X Hu,B M Revelle,T P Kogan,I L Scott,R A Dixon,E T Yeh,P J Beck

Blood 78:805-11 PubMed1713515

1991

Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

T K Kishimoto,R A Warnock,M A Jutila,E C Butcher,C Lane,D C Anderson,C W Smith

Proceedings of the National Academy of Sciences of the United States of America 87:2244-8 PubMed2179952

1990

Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.

Applications

Unspecified application

Species

Unspecified reactive species

T K Kishimoto,M A Jutila,E C Butcher
View all publications
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