AB222

FITC Anti-CD62L antibody [FMC46]

Name: FITC Anti-CD62L antibody [FMC46] (ab222) | Abcam
Brand: Abcam Limited
SKU: AB222
Price: 250 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD62L antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Monkey samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing SELL protein.

View Alternative Names

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1

1 Images

Flow Cytometry - FITC Anti-CD62L antibody [FMC46] (AB222)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD62L antibody [FMC46] (AB222)

ab183 staining CD62L (FITC) in human peripheral blood granulocytes by Flow Cytometry analysis.

578 PE

PE Anti-CD62L antibody [FMC46]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FMC46

Isotype

IgG2b

Light chain type

unknown

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Monkey

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing SELL protein. The exact immunogen used to generate this antibody is proprietary information.

P14151

Specificity

FITC.FMC46 recognises the human CD62L cell surface antigen, a 150 kD glycoprotein also known as L-selectin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 10 μl to label 106 cells or 100 μl whole blood.<a href='/en-us/products/primary-antibodies/fitc-mouse-igg2b-plpv219-isotype-control-ab91368'>ab91368</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody." }, "Monkey": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 10 μl to label 106 cells or 100 μl whole blood.<a href='/en-us/products/primary-antibodies/fitc-mouse-igg2b-plpv219-isotype-control-ab91368'>ab91368</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody." } } }

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Product details

Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).

See full target information SELL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 12:4529-35 PubMed16874866

2006

Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Tso Lin,Shih-Hwa Chiou,Chung-Lan Kao,Yi-Ming Shyr,Chien-Jen Hsu,Yih-Wen Tarng,Larry L-T Ho,Ching-Fai Kwok,Hung-Hai Ku

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-CD62L antibody [FMC46]

Flow Cytometry - FITC Anti-CD62L antibody [FMC46] (AB222)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise