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AB24876

FITC Anti-CD62L antibody [MEL-14]

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(3 Publications)

Rat Monoclonal CD62L antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD62L protein.
1 Images
Flow Cytometry - FITC Anti-CD62L antibody [MEL-14] (AB24876)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD62L antibody [MEL-14] (AB24876)

Flow cytometry C57Bl/6 splenocytes labeling CD62L with ab24876 at 0.25μg (solid line), or Rat IgG2a isotype control at 0.25μg (dashed line).

  • Unconjugated

    Anti-CD62L antibody [MEL-14]

  • redFluor™ 710

    redFluor™ 710 Anti-CD62L antibody [MEL-14]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD62L antibody [MEL-14]

  • Biotin

    Biotin Anti-CD62L antibody [MEL-14]

  • 660 APC

    APC Anti-CD62L antibody [MEL-14]

  • Carrier free

    Anti-CD62L antibody [MEL-14] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MEL-14

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CD62L
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Immunotherapy 16:975-985 PubMed39115961

2024

The development of anti-PD-1 antibody-induced spinal cord injury in bone marrow transplant C57BL/6 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Huachun Chen,Zhouxiao Lu,Xiaowei Ni,Hui Zhang,Guiyuan Chen,Xiaoyu Wu,Mingxing Ding

Journal of immunology (Baltimore, Md. : 1950) 185:2737-46 PubMed20660707

2010

TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection.

Applications

Flow Cyt

Species

Mouse

Vicky L Morrison,Tom A Barr,Sheila Brown,David Gray

Journal of immunology (Baltimore, Md. : 1950) 136:3529-34 PubMed3084633

1986

Dual immunofluorescence studies of cortisone-induced thymic involution: evidence for a major cortical component to cortisone-resistant thymocytes.

Applications

Unspecified application

Species

Unspecified reactive species

R A Reichert,I L Weissman,E C Butcher
View all publications
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