AB108949

FITC Anti-CD63 antibody [AD1]

Name: Anti-CD63 FITC antibody [AD1] (ab108949) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB108949

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal CD63 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd63 protein.

View Alternative Names

CD63, CD63 antigen, Mast cell antigen AD1, Cd63

1 Images

Flow Cytometry - FITC Anti-CD63 antibody [AD1] (AB108949)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD63 antibody [AD1] (AB108949)

ab108949 detecting CD63 in Rat peripheral blood platelets by Flow Cytometric analysis.

Unconjugated

Anti-CD63 antibody [AD1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd63 protein. The exact immunogen used to generate this antibody is proprietary information.

P28648

Specificity

Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1, and that clone AD1 partially inhibits IgE-mediated histamine release

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli (By similarity).

See full target information Cd63

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 30:2257-2273 PubMed35278675

2022

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Ottaviani,Rio P Juni,Ricardo C de Abreu,Marida Sansonetti,Vasco Sampaio-Pinto,Julie Halkein,Jana C Hegenbarth,Nadja Ring,Kevin Knoops,Jordy M M Kocken,Carlos de Jesus,Auriane C Ernault,Hamid El Azzouzi,Frank Rühle,Servé Olieslagers,Hugo Fernandes,Lino Ferreira,Luca Braga,Monika Stoll,Diana S Nascimento,Leon J de Windt,Paula A da Costa Martins

The Journal of biological chemistry 266:1903-9 PubMed1703158

1991

A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.

Applications

WB, IP

Species

Rat, Rat

S Kitani,E Berenstein,S Mergenhagen,P Tempst,R P Siraganian

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD63 antibody [AD1]

Flow Cytometry - FITC Anti-CD63 antibody [AD1] (AB108949)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise