AB18235

FITC Anti-CD63 antibody [MEM-259]

Name: Anti-CD63 FITC antibody [MEM-259] (ab18235) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB18235
Price: 95 USD
Availability: InStock

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(15 Publications)

Mouse Monoclonal CD63 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Cell preparation containing CD63 protein.

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

1 Images

Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (AB18235)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (AB18235)

ab18235 staining CD63 in human peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, analysed by Flow Cytometry.

Unconjugated

Anti-CD63 antibody [MEM-259]
578 PE

PE Anti-CD63 antibody [MEM-259]
667 PE/Cy5®

PE/Cy5® Anti-CD63 antibody [MEM-259]
675 PerCP

PerCP Anti-CD63 antibody [MEM-259]
Biotin

Biotin Anti-CD63 antibody [MEM-259]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-259

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/5", "FlowCyt-species-notes": "<p>20 μl for a 100 μl sample is recommended.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene CD63 functions as a cell surface receptor for TIMP1 and is involved in the activation of various cellular signaling cascades. It plays a role in the activation of ITGB1 and integrin signaling, which leads to the activation of AKT, FAK/PTK2, and MAP kinases, promoting cell survival, actin cytoskeleton reorganization, and cell adhesion, spreading, and migration through the activation of AKT and FAK/PTK2. Additionally, it participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2. CD63 is involved in intracellular vesicular transport processes and is essential for the normal trafficking of the PMEL luminal domain, crucial for melanocyte development and maturation. It also contributes to leukocyte adhesion onto endothelial cells by regulating SELP trafficking. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in response to other stimuli. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD63

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:10964 PubMed38744871

2024

Stem cells derived exosomes as biological nano carriers for VCR sulfate for treating breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed H Farouk,Ahmed Aref,Belal A Fathy,Ahmed N Abdallah

Journal of orthopaedic surgery and research 18:892 PubMed37993925

2023

Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Meng,Yun Yang,Wei Feng,Penglei Ma,Rui Bai

International journal of molecular sciences 24: PubMed37108601

2023

Extracellular Vesicles Secreted by Pre-Hatching Bovine Embryos Produced In Vitro and In Vivo Alter the Expression of IFNtau-Stimulated Genes in Bovine Endometrial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Constanza Aguilera,Alejandra Estela Velásquez,Miguel Angel Gutierrez-Reinoso,Yat Sen Wong,Barbara Melo-Baez,Joel Cabezas,Diego Caamaño,Felipe Navarrete,Daniela Rojas,Gonzalo Riadi,Fidel Ovidio Castro,Llretny Rodriguez-Alvarez

Journal of cellular and molecular medicine 26:2620-2632 PubMed35419917

2022

Overexpression of FOXD2-AS1 enhances proliferation and impairs differentiation of glioma stem cells by activating the NOTCH pathway via TAF-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Yanli Cheng,Qi Yang,Lei Kuang,Guolei Liu

Cells 10: PubMed34572021

2021

A New Approach for Prostate Cancer Diagnosis by miRNA Profiling of Prostate-Derived Plasma Small Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Zabegina,Inga Nazarova,Nadezhda Nikiforova,Maria Slyusarenko,Elena Sidina,Margarita Knyazeva,Evgenia Tsyrlina,Sergey Novikov,Sergey Reva,Anastasia Malek

Molecules (Basel, Switzerland) 26: PubMed33921245

2021

Effects of Induced Exosomes from Endometrial Cancer Cells on Tumor Activity in the Presence of Extract.

Applications

Unspecified application

Species

Unspecified reactive species

Yoonjin Park,Kyunghwa Lee,Suhng Wook Kim,Min Woo Lee,Boyong Kim,Seung Gwan Lee

Polymers 13: PubMed33572666

2021

Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Slyusarenko,Nadezhda Nikiforova,Elena Sidina,Inga Nazarova,Vladimir Egorov,Yuri Garmay,Anastasiia Merdalimova,Natalia Yevlampieva,Dmitry Gorin,Anastasia Malek

International journal of molecular sciences 21: PubMed33255183

2020

MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Melo-Baez,Yat S Wong,Constanza J Aguilera,Joel Cabezas,Ana C F Mançanares,Gonzalo Riadi,Fidel O Castro,Lleretny Rodriguez-Alvarez

Reproduction (Cambridge, England) 158:477-492 PubMed31600718

2019

Extracellular vesicles secreted during blastulation show viability of bovine embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin A Mellisho,Mario A Briones,Alejandra E Velásquez,Joel Cabezas,Fidel O Castro,Lleretny Rodríguez-Álvarez

Cellular and molecular life sciences : CMLS 77:937-952 PubMed31312880

2019

Knockout of beta-2 microglobulin enhances cardiac repair by modulating exosome imprinting and inhibiting stem cell-induced immune rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Lianbo Shao,Yu Zhang,Xiangbin Pan,Bin Liu,Chun Liang,Yuqing Zhang,Yanli Wang,Bing Yan,Wenping Xie,Yi Sun,Zhenya Shen,Xi-Yong Yu,Yangxin Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD63 antibody [MEM-259]

Flow Cytometry - FITC Anti-CD63 antibody [MEM-259] (AB18235)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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