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AB323935

FITC Anti-CD68 antibody [EPR23917-164]

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Rabbit Recombinant Monoclonal CD68 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

View Alternative Names

CD68, Macrosialin, Cd68

2 Images
Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)

Flow cytometry staining of C57 BL/6 Mouse Bone Marrow Cell-derived Macrophages with ab323935 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323935 or Rabbit IgG [EPR25A] FITC (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C. The cells were simultaneously stained with F4/80.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [EPR23917-164] (AB323935)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab323935 (right) or Rabbit IgG [EPR25A] FITC (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323935 or Rabbit IgG [EPR25A] FITC (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C. The cells were simultaneously stained with F4/80.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

  • Carrier free

    Anti-CD68 antibody [EPR23917-164] - BSA and Azide free

  • Unconjugated

    Anti-CD68 antibody [EPR23917-164]

  • 660 APC

    APC Anti-CD68 antibody [EPR23917-164]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD68 antibody [EPR23917-164]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD68 antibody [EPR23917-164]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD68 antibody [EPR23917-164]

  • 578 PE

    PE Anti-CD68 antibody [EPR23917-164]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23917-164

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Mouse, Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Mouse unsuitable for IHC-Fr application

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Complementary Products

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information Cd68
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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