AB134351

FITC Anti-CD68 antibody [Y1/82A]

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(5 Publications)

Mouse Monoclonal CD68 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CD68.

View Alternative Names

CD68, Macrosialin, Gp110

1 Images

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [Y1/82A] (AB134351)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [Y1/82A] (AB134351)

Intracellular immunofluorescent staining with flow cytometric analysis labelling CD68 in Human peripheral blood monocytes intracellularly stained by ab134351.

Unconjugated

Anti-CD68 antibody [Y1/82A]
Biotin

Biotin Anti-CD68 antibody [Y1/82A]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Y1/82A

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human CD68. The exact immunogen used to generate this antibody is proprietary information.

P34810

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "5 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p>in 100 μl staining volume. Or Use 5 μl for 100 μl of whole blood.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2b-kappa-monoclonal-mg2b-57-isotype-control-ab18427'>ab18427</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

The reagent is free of unconjugated FITC.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Purified by affinity chromatography. Purity of this monoclonal antibodies is 95%.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 98% PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information CD68

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:9548-9563 PubMed35387564

2022

Emodin suppresses hepatocellular carcinoma growth by regulating macrophage polarization via microRNA-26a/transforming growth factor beta 1/protein kinase B.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Yin,Xiansheng Zhao,Xuejiao Chen,Guanxin Shen

Aging 12:6928-6946 PubMed32291381

2020

AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Song Gu,Hanhao Dai,Xilian Zhao,Chang Gui,Jianchao Gui

Clinical science (London, England : 1979) 133:2265-2282 PubMed31657855

2019

Tumor-associated macrophage-derived exosomal microRNA-155-5p stimulates intracranial aneurysm formation and macrophage infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengzhe Feng,Xiaoxi Zhang,Li Li,Chuanchuan Wang,Mingtao Feng,Kaijun Zhao,Rui Zhao,Jianmin Liu,Yibin Fang

The Journal of experimental medicine 216:337-349 PubMed30610104

2019

Phosphatase Shp2 exacerbates intestinal inflammation by disrupting macrophage responsiveness to interleukin-10.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xiao,Huilun Zhang,Yu Zhang,Mingzhu Zheng,Rongbei Liu,Yuan Zhao,Xue Zhang,Hongqiang Cheng,Qian Cao,Yuehai Ke

Respiratory research 18:127 PubMed28646872

2017

Role of P2XR in the development and progression of pulmonary hypertension.

Applications

IHC-P

Species

Rat

Jie Yin,Shuling You,Haopeng Liu,Li Chen,Chengdong Zhang,Hesheng Hu,Mei Xue,Wenjuan Cheng,Ye Wang,Xinran Li,Yugen Shi,Nannan Li,Suhua Yan,Xiaolu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD68 antibody [Y1/82A]

Flow Cytometry (Intracellular) - FITC Anti-CD68 antibody [Y1/82A] (AB134351)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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