AB239246

FITC Anti-CD73 antibody [AD2]

Name: Anti-CD73 FITC antibody [AD2] (ab239246) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB239246
Price: 135 USD
Availability: InStock

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(12 Publications)

Mouse Monoclonal CD73 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 12 publications.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

1 Images

Flow Cytometry - FITC Anti-CD73 antibody [AD2] (AB239246)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD73 antibody [AD2] (AB239246)

Flow cytometric analysis of human peripheral blood labeling CD73 with ab239246. Surface staining. Gated on leukocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD2

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).

See full target information NT5E

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 25:35-48 PubMed38058606

2023

Epigenetic mechanism of miR-26b-5p-enriched MSCs-EVs attenuates spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Zhenxiao Ren,Tianzuo Niu,Siyuan Li

Stem cells international 2022:2164057 PubMed36311041

2022

miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baodong Wang,Na Xu,Li Cao,Xiaojun Yu,Shanxi Wang,Qikun Liu,Yinguang Wang,Haoran Xu,Yang Cao

Cell death & disease 13:535 PubMed35672285

2022

Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Jun Wang,Zhen-Zhen Qiu,Fu-Wei Chen,An-Li Mao,Jun-Chao Bai,Ye-Jing Hong,Zhong-Pan Zhang,Wu-An Zhu,Zhi-Wei Zhang,Hao Zhou

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 166:161-167 PubMed34747415

2021

A comparative study on the secretion of various cytokines by pulp stem cells at different passages and their neurogenic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Yan,Ola A Nada,Ling-Ling Fu,Dong-Zhen Li,Hong-Chao Feng,Li-Ming Chen,Martin Gosau,Reinhard E Friedrich,Ralf Smeets

Cell death discovery 7:224 PubMed34455417

2021

Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shui Ma,Ji-Bin Liu,Lan Lin,Hui Zhang,Jian-Jun Wu,Yi Shi,Cheng-You Jia,Dan-Dan Zhang,Fei Yu,Hui-Min Wang,Yu-Zhen Yin,Xiao-Hui Jiang,Pei-Yao Wang,Lin-Lin Tian,Ping-Sheng Cao,Xu-Ming Wu,Hai-Min Lu,Li-Peng Gu,Jia-Jia Zhang,Gu-Jun Cong,Pei Luo,Xiao-Ming Zhong,Bo Cai,Min-Xin Shi,Su-Qing Zhang,Liu Li,Wen-Jie Zhang,Yu Liu,Zhi-Zhen Li,Ting-Miao Wu,Zhi-Jun Wu,Gao-Ren Wang,Zhong-Wei Lv,Chang-Chun Ling,Kai-Jian Chu,Da Fu

Therapeutic advances in chronic disease 12:20406223211007369 PubMed33995992

2021

MicroRNA-21 from bone marrow mesenchymal stem cell-derived extracellular vesicles targets TET1 to suppress KLF4 and alleviate rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Qing Li,Yu-Xuan Fang,Ying Liu,Fan-Ru Meng,Xia Wu,Chun-Wang Zhang,Yu Zhang,Yan-Qing Liu,Dan Liu

Journal of cellular and molecular medicine 25:1867-1883 PubMed33417281

2021

Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Meng,Baofang Zhang,Yingming Zhang,Shuyan Wang,Xiaohui Zhu

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia

Stem cell research & therapy 11:455 PubMed33109266

2020

microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ding,Jinhua Liu,Xiaofei Zhang

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

View all publications

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Product promise

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FITC Anti-CD73 antibody [AD2]

Flow Cytometry - FITC Anti-CD73 antibody [AD2] (AB239246)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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