AB24910

FITC Anti-CD8 alpha antibody [3-298]

Name: Anti-CD8 alpha FITC antibody [3-298] (ab24910) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB24910
Price: 510 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD8 alpha antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Chicken samples. Cited in 1 publication.

View Alternative Names

CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8A antigen, CD8A molecule, CD8A_HUMAN, CD_antigen=CD8a, LYT3, Leu2, Leu2 T lymphocyte antigen, Ly 2, Ly 35, Ly B, Ly3, Lyt 2.1 lymphocyte differentiation antigen (AA at 100), OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt 2, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen, alpha polypeptide (p32), p32

1 Images

Flow Cytometry - FITC Anti-CD8 alpha antibody [3-298] (AB24910)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD8 alpha antibody [3-298] (AB24910)

Chicken peripheral blood lymphocytes were costained using CD8 alpha-FITC and CD4-PE to distinguish cell subpopulations. Cells were incubated with anti-CD8 alpha antibody [3-298] (FITC) (ab24910) at 1 μg/10⁶ cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3-298

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Chicken

Applications

Flow Cyt

applications

Specificity

This antibody recognises CD8a.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chicken": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Turkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

In chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha and CD8 beta chains, a relatively large proportion of the CD8+ TCR gamma delta cells in the spleens of embryos and young chicks express only the alpha chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha's role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha's connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD8A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 11:439-447 PubMed26893628

2016

Biological characterization of metanephric mesenchymal stem cells from the Beijing duck.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Yabin Pu,Yujiao Sun,Ping Zhang,Qian Li,Kunfu Wang,Wenjie Wang,Yuehui Ma,Weijun Guan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-CD8 alpha antibody [3-298]

Flow Cytometry - FITC Anti-CD8 alpha antibody [3-298] (AB24910)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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