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AB326493

FITC Anti-CD83 antibody [HB15e]

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Mouse Monoclonal CD83 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD83, CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15

3 Images
Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)

Separation of human CD83 positive lymphocytes (red-filled) from CD83 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human interferon gamma stimulated peripheral blood mononuclear cells stained using ab326493 (concentration in sample 3 μg/ml).

Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)

Flow cytometry surface staining pattern of human interferon gamma stimulated peripheral blood mononuclear cells stained using ab326493 (concentration in sample 3 μg/ml).

Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD83 antibody [HB15e] (AB326493)

Flow cytometry multicolor surface staining pattern of Side Scatter low events of human interferon gamma stimulated peripheral blood mononuclear cells stained using anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and ab326493 (concentration in sample 3 μg/ml).

  • Carrier free

    Anti-CD83 antibody [HB15e] - BSA and Azide free

  • Unconjugated

    Anti-CD83 antibody [HB15e]

  • 660 APC

    APC Anti-CD83 antibody [HB15e]

  • 578 PE

    PE Anti-CD83 antibody [HB15e]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD83 antibody [HB15e]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD83 antibody [HB15e]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD83 antibody [HB15e]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HB15e

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHCII trafficking MARCHF8. Also inhibits MARCHF1 association with MHCII or CD86 to prevent their ubiquitination and subsequent degradation (PubMed : 21220452). In addition, acts as an important modulator of protective responses against acute infections (By similarity).
See full target information CD83
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Product promise

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