AB77276

FITC Anti-CD86 antibody [BU63]

Name: FITC Anti-CD86 antibody [BU63] (ab77276) | Abcam
Brand: Abcam Limited
SKU: AB77276
Price: 95 USD
Availability: InStock

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(14 Publications)

Mouse Monoclonal CD86 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Cell preparation containing CD86 protein.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

2 Images

Flow Cytometry - FITC Anti-CD86 antibody [BU63] (AB77276)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD86 antibody [BU63] (AB77276)

Separation of human monocytes (red-filled) from CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD86 antibody [BU63] (AB77276)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).

Carrier free

Anti-CD86 antibody [BU63] - BSA and Azide free
Unconjugated

Anti-CD86 antibody [BU63]
667 PE/Cy5®

PE/Cy5® Anti-CD86 antibody [BU63]
675 PerCP

PerCP Anti-CD86 antibody [BU63]
778 PE/Cy7®

PE/Cy7® Anti-CD86 antibody [BU63]
660 APC

APC Anti-CD86 antibody [BU63]
578 PE

PE Anti-CD86 antibody [BU63]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BU63

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD86 protein. The exact immunogen used to generate this antibody is proprietary information.

P42081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 20 μl per 100 μl of whole blood or 10<sup>6</sup> cells</p>" } } }

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Product details

The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

See full target information CD86

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

The clinical respiratory journal 19:e70079 PubMed40329406

2025

USP7-Mediated ICAM1 Facilitates Lipopolysaccharide-Induced Human Pulmonary Microvascular Endothelial Cell Injury to Accelerate Pediatric Acute Respiratory Distress Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Jing Wu,Lili Zhao,Lian Liu

Communications biology 8:514 PubMed40155473

2025

Defining gastric cancer ecology: the crucial roles of TREM2 macrophages and fibroblasts in tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cao,Dianshui Sun,Can Tu,Jihua Wang,Runjia Fu,Rumei Gong,Yueying Xiao,Qin Liu,Xiaomei Li

Thoracic cancer 15:227-238 PubMed38087801

2023

Tumor-related exosomal circ_0001715 promotes lung adenocarcinoma cell proliferation and metastasis via enhancing M2 macrophage polarization by regulating triggering receptor expressed on myeloid cells-2.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Chen,Chengzhang Cao,Jun Ma

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang

Frontiers in bioengineering and biotechnology 11:1173169 PubMed37214283

2023

Low-intensity pulsed ultrasound reduces lymphedema by regulating macrophage polarization and enhancing microcirculation.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Liu,Jia Li,Yu Bian,Xiaojie Zhang,Xiaojun Cai,Yuanyi Zheng

Journal for immunotherapy of cancer 11: PubMed37094986

2023

YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6.

Applications

Unspecified application

Species

Unspecified reactive species

Saisai Chen,Kai Lu,Yue Hou,Zonghao You,Chuanjun Shu,Xiaoying Wei,Tiange Wu,Naipeng Shi,Guangyuan Zhang,Jianping Wu,Shuqiu Chen,Lihua Zhang,Wenchao Li,Dingxiao Zhang,Shenghong Ju,Ming Chen,Bin Xu

World journal of clinical cases 9:2181-2191 PubMed33869594

2021

Iguratimod promotes transformation of mononuclear macrophages in elderly patients with rheumatoid arthritis by nuclear factor-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Liu,Li-Ping Song,Rong-Bin Li,Le-Heng Feng,Hui Zhu

PLoS pathogens 17:e1009091 PubMed33411730

2021

Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.

Applications

Unspecified application

Species

Unspecified reactive species

Prabha Shrestha,David A Davis,Hannah K Jaeger,Alexandra Stream,Ashley I Aisabor,Robert Yarchoan

Evidence-based complementary and alternative medic 2021:8868527 PubMed33505510

2021

Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-B Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Bingfeng Lin,Zhifeng Li,Jie Su,Yulin Feng

Free radical biology & medicine 141:10-20 PubMed31153975

2019

Y-box binding protein 1 regulates ox-LDL mediated inflammatory responses and lipid uptake in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xueming Cao,Na Zhu,Li Li,Yuwei Zhang,Yan Chen,Jing Zhang,Jiang Li,Chuanyu Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD86 antibody [BU63]

Flow Cytometry - FITC Anti-CD86 antibody [BU63] (AB77276)

Flow Cytometry - FITC Anti-CD86 antibody [BU63] (AB77276)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise