AB25672

FITC Anti-CD90 / Thy1 antibody [G7]

Name: Anti-CD90 / Thy1 FITC antibody [G7] (ab25672) Rat monoclonal IgG2c | Abcam
Brand: Abcam Limited
SKU: AB25672
Price: 525 USD
Availability: InStock

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(11 Publications)

Rat Monoclonal CD90 / Thy1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 11 publications.

View Alternative Names

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

2 Images

Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [G7] (AB25672)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [G7] (AB25672)

Flow cytometry analysis of BALB/c mouse splenocytes stained with Rat Anti-Mouse CD90-FITC ab25641 (1 µg/10⁶cells) and Rat Anti-Mouse CD3 epsilon-PE ab25641.

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [G7] (AB25672)

ab25672, at a concentration of 1µg for 10⁶ cells, staining CD90/Thy1 in BALB/c mice mesenteric lymph node cells by Immunofluorescence. Some cells were also stained with rat IgG2c FITC as a negative control. Lymphocytes were then gated and analyzed on a flow cytometer.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

Isotype

IgG2c

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Epitope

This antibody reacts with CD90/Thy1 epitope region A.

Specificity

This antibody recognises CD90/Thy1, alloantigens Thy1.1 and Thy1.2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2c-kappa-rtk4174-isotype-control-ab114134'>ab114134</a> - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

This antibody has been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information Thy1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 10:rbad011 PubMed36852397

2023

Selenium-modified bone cement promotes osteoporotic bone defect repair in ovariectomized rats by restoring GPx1-mediated mitochondrial antioxidant functions.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Zhou,Weikai Chen,Chao Gu,Hao Liu,Xiayu Hu,Lei Deng,Wei He,Yong Xu,Xuesong Zhu,Huilin Yang,Xi Chen,Fan He,Tao Liu

Stem cells international 2023:7545635 PubMed36644009

2023

Molecular Mechanism of Long Noncoding RNA SNHG14 in Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells through the NEDD4L/FOXA2/PCP4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wang,Mingxing Fan,Yan An,Da He

Oxidative medicine and cellular longevity 2022:8825784 PubMed35281474

2022

BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP Delivery of miR-877-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Feng,Zhengwei Li,Liang Yang,Haidong Liang,Hongtao He,Lin Liu,Wei Zhang

Cell death discovery 8:2 PubMed35013126

2022

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhang Fan,Shiming Li,Dawei Wang

Theranostics 10:17-35 PubMed31903103

2020

Macrophage MSR1 promotes BMSC osteogenic differentiation and M2-like polarization by activating PI3K/AKT/GSK3β/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Jie Zhao,Fan-Qi Kong,Jian Jie,Qing Li,Hao Liu,An-Di Xu,Ya-Qing Yang,Bin Jiang,Dong-Dong Wang,Zhong-Qiu Zhou,Peng-Yu Tang,Jian Chen,Qian Wang,Zheng Zhou,Qi Chen,Guo-Yong Yin,Han-Wen Zhang,Jin Fan

Journal of cellular physiology 234:23485-23494 PubMed31206187

2019

Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guangjie Li,Jiangdong An,Xingwen Han,Xueliang Zhang,Wenjin Wang,Shuanke Wang

Tissue & cell 56:23-30 PubMed30736901

2019

Adipose derived mesenchymal stem cells improve the structure and function of autografted mice ovaries through reducing oxidative stress and inflammation: A stereological and biochemical analysis.

Applications

Unspecified application

Species

Unspecified reactive species

E Shojafar,M Soleimani Mehranjani,S M A Shariatzadeh

Cytotherapy 20:1324-1336 PubMed30360962

2018

Adipose-derived mesenchymal stromal cell transplantation at the graft site improves the structure and function of autografted mice ovaries: a stereological and biochemical analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Shojafar,Malek Soleimani Mehranjani,Seyed Mohammad Ali Shariatzadeh

Cell biology international 42:570-579 PubMed29314362

2018

A small population of resident limb bud mesenchymal cells express few MSC-associated markers, but the expression of these markers is increased immediately after cell culture.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Cristina Marín-Llera,Jesús Chimal-Monroy

PloS one 11:e0163876 PubMed27695046

2016

Role of Dicer1-Dependent Factors in the Paracrine Regulation of Epididymal Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Jerczynski,Nicolas Lacroix-Pépin,Eric Boilard,Ezequiel Calvo,Agathe Bernet,Michel A Fortier,Ida Björkgren,Petra Sipilä,Clémence Belleannée

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-CD90 / Thy1 antibody [G7]

Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [G7] (AB25672)

Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [G7] (AB25672)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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