FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

5 product images

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  Immunocytochemistry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  ab226 staining CD90 / Thy1 in undifferentiated PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI(shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

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  Immunocytochemistry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 10⁶ in 100μl at 1μg/ml (1/500)) for 30 min at 22°C.
  

  Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This antibody gave a positive signal in PC12 (undifferentiated) cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 10⁶ in 100μl at 1μg/ml (1/500)) for 30 min on ice.
  

  Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• 

  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (FITC Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype control ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.